| 1 | Jildou Schaaf (Neo) | NED | 41.05 | SB | - |
| 2 | Jasmijn ter Haar (B1) | NED | 41.35 | SB | +0.300 |
| 3 | Cerinthe de Jonge (Neo) | NED | 41.44 | | +0.390 |
| 4 | Claretta Caravita (Senior) | NED | 41.54 | SB | +0.490 |
| 5 | Amée Hollanders (Neo) | NED | 41.55 | SB | +0.500 |
| 6 | Lieke Hijlkema (A1) | NED | 41.89 | SB | +0.840 |
| 7 | Nynke Tinga (A2) | NED | 41.94 | PB | +0.890 |
| 8 | Iris Willemsen (Senior) | NED | 42.40 | SB | +1.350 |
| 9 | Maaike Koelewijn (A1) | NED | 42.42 | | +1.370 |
| 10 | Emma Hulshof (Senior) | NED | 42.54 | | +1.490 |
| 11 | Gwen Konter (Neo) | NED | 42.69 | PB | +1.640 |
| 12 | Mijke Anne Kanneworff (M40) | NED | 42.85 | SB | +1.800 |
| 13 | Marloes Westerhof (M40) | NED | 42.96 | | +1.910 |
| 14 | Lien van Dasselaar (Neo) | NED | 43.01 | SB | +1.960 |
| 15 | Britt Post (B1) | NED | 43.06 | PB | +2.010 |
| 16 | Nanette de Boon (C1) | NED | 43.14 | PB | +2.090 |
| 17 | Lauren van den Brink (D2) | NED | 43.40 | | +2.350 |
| 18 | Liv Kogelman (C2) | NED | 43.74 | SB | +2.690 |
| 19 | Renske van Winkoop (D2) | NED | 43.80 | | +2.750 |
| 20 | Claudia Henckel (M35) | NED | 44.08 | SB | +3.030 |
| 21 | Lieve Bunt (A2) | NED | 44.24 | SB | +3.190 |
| 22 | Lenthe Jansen (A1) | NED | 44.35 | PB | +3.300 |
| 23 | Britt Steg (B2) | NED | 45.05 | | +4.000 |
| 24 | Marjolein Ooms (C2) | NED | 45.11 | PB | +4.060 |
| 25 | Tessel Huizinga (C2) | NED | 45.12 | PB | +4.070 |
| 26 | Eva Weultjes (B2) | NED | 45.35 | SB | +4.300 |
| 27 | Nynke Lankhaar (A1) | NED | 45.72 | SB | +4.670 |
| Maaike Helleman (C2) | NED | 45.72 | SB | +4.670 |
| 29 | Jeanette Hutterd ten Hag (M45) | NED | 45.78 | SB | +4.730 |
| 30 | Nynke Krol (D2) | NED | 45.83 | SB | +4.780 |
| 31 | Lieke van Ooij (A1) | NED | 45.98 | PB | +4.930 |
| 32 | Zoè de Esch (C2) | NED | 46.24 | | +5.190 |
| 33 | Madee Hadders (D2) | NED | 46.26 | PB | +5.210 |
| 34 | Shirley Jaarsma (M40) | NED | 46.50 | SB | +5.450 |
| 35 | Janette de Groot-Bron (M45) | NED | 47.18 | SB | +6.130 |
| 36 | Florine Zunnebeld (D2) | NED | 50.86 | | +9.810 |
| 37 | Marije Kerkhof (M50) | NED | 51.64 | SB | +10.590 |
| 38 | Bente van Dasselaar (C2) | NED | 51.78 | SB | +10.730 |
| 39 | Avani Sudarshan Mogasale (n/a) | IND | 58.19 | PB | +17.140 |
| 40 | Aayanika Banerjee (n/a) | IND | 1:05.05 | PB | +24.000 |
| 41 | Sanya Kalaria (E2) | IND | 1:07.06 | PB | +26.010 |