| 1 | Marrit Fledderus (Senior) | NED | 38.20 | SB | - |
| 2 | Pien Hersman (Neo) | NED | 38.79 | | +0.590 |
| 3 | Isabel Grevelt (Neo) | NED | 39.00 | SB | +0.800 |
| 4 | Sacha van der Weide (Neo) | NED | 39.12 | | +0.920 |
| 5 | Henny de Vries (Neo) | NED | 40.09 | | +1.890 |
| 6 | Leonie Bats (Neo) | NED | 40.82 | | +2.620 |
| 7 | Jasmijn Veenhuis (A1) | NED | 40.94 | SB | +2.740 |
| 8 | Elanne de Vries (A2) | NED | 40.98 | PB | +2.780 |
| 9 | Britt Breider (A1) | NED | 41.58 | PB | +3.380 |
| 10 | Ance Kucina (A2) | LAT | 41.99 | SB | +3.790 |
| 11 | Julia Pereira de Vos (A2) | BRA | 42.04 | | +3.840 |
| 12 | Naomi van der Werf (Senior) | NED | 42.10 | SB | +3.900 |
| 13 | Karlijn Schellekens (A2) | NED | 42.54 | SB | +4.340 |
| 14 | Amber Koetsier (B2) | NED | 43.26 | PB | +5.060 |
| 15 | Ymke Wubben (B2) | NED | 43.43 | SB | +5.230 |
| 16 | Maud Groenen (B1) | NED | 43.79 | SB | +5.590 |
| 17 | Amina Dillmann (B1) | NED | 44.08 | PB | +5.880 |
| 18 | Karlijn te Poele (C1) | NED | 44.61 | PB | +6.410 |
| 19 | Anouk Könst (A2) | NED | 44.66 | SB | +6.460 |
| 20 | Martha Trude Brehmer (C2) | SWE | 44.88 | SB | +6.680 |
| 21 | Youssra Pals (B1) | NED | 45.16 | PB | +6.960 |
| 22 | Hiba Pals (C1) | NED | 47.47 | SB | +9.270 |
| WDR | Sylke Kas (Neo) | NED | WDR | | - |
| WDR | Fleur Smits (A1) | NED | WDR | | - |
| WDR | Maud Blokhorst (B2) | NED | WDR | | - |