| 1 | Yuuma Murakami (Senior) | JPN | 34.74 | | - |
| 2 | Tatsuya Shinhama (Senior) | JPN | 35.04 | | +0.300 |
| 3 | Daichi Yamanaka (Senior) | JPN | 35.26 | | +0.520 |
| 4 | Yuuto Fujino (Senior) | JPN | 35.31 | | +0.570 |
| 5 | Takuya Morimoto (Senior) | JPN | 35.32 | | +0.580 |
| 6 | Katsuhiro Kuratsubo (Neo) | JPN | 35.40 | | +0.660 |
| 7 | Tsubasa Hasegawa (Senior) | JPN | 35.43 | | +0.690 |
| 8 | Souki Ooshima (Neo) | JPN | 35.44 | SB | +0.700 |
| 9 | Shunsuke Nakamura (Senior) | JPN | 35.45 | | +0.710 |
| 10 | Wataru Morishige (Neo) | JPN | 35.47 | | +0.730 |
| 11 | Hirokazu Kobayashi (Senior) | JPN | 35.51 | | +0.770 |
| 12 | Joji Kato (Senior) | JPN | 35.52 | | +0.780 |
| 13 | Itsushi Tsujimoto (Senior) | JPN | 35.57 | | +0.830 |
| 14 | Keigo Abe (Senior) | JPN | 35.74 | | +1.000 |
| 15 | Ryota Kojima (Neo) | JPN | 35.77 | | +1.030 |
| 16 | Ryouhei Haga (Senior) | JPN | 35.78 | | +1.040 |
| 17 | Yuuma Uehara (Senior) | JPN | 35.81 | | +1.070 |
| 18 | Tsukasa Oowada (Senior) | JPN | 35.82 | | +1.080 |
| 19 | Kouki Kubo (Neo) | JPN | 36.00 | | +1.260 |
| 20 | Kyouhei Mukaiyama (Neo) | JPN | 36.05 | | +1.310 |
| 21 | Masayuki Ishikawa (Senior) | JPN | 36.09 | SB | +1.350 |
| 22 | Eikichi Sakamoto (Senior) | JPN | 36.22 | | +1.480 |
| 23 | Motoki Abe (B2) | JPN | 36.27 | | +1.530 |
| 24 | Ryuu Kosaka (Senior) | JPN | 36.31 | SB | +1.570 |
| 25 | Reima Ugajin (Neo) | JPN | 36.32 | | +1.580 |
| 26 | Rio Iwasa (Neo) | JPN | 36.35 | | +1.610 |
| 27 | Fuuta Kiriki (A2) | JPN | 36.40 | | +1.660 |
| 28 | Shouta Tsubaki (Neo) | JPN | 36.41 | | +1.670 |
| 29 | Kiyotaka Tokiyasu (B2) | JPN | 36.44 | SB | +1.700 |
| 30 | Junmu Hara (A2) | JPN | 36.45 | | +1.710 |
| 31 | Keisuke Tsuchiya (A1) | JPN | 36.55 | | +1.810 |
| 32 | Shane Williamson (Senior) | JPN | 36.59 | | +1.850 |
| 33 | Kazuki Sakakibara (Neo) | JPN | 36.74 | | +2.000 |
| 34 | Yuuta Hirose (B1) | JPN | 36.93 | | +2.190 |
| 35 | Takahiro Kawamori (Neo) | JPN | 36.96 | | +2.220 |
| 36 | Jin Akasaka (Neo) | JPN | 37.12 | | +2.380 |
| 37 | Shinnosuke Honma (Neo) | JPN | 37.17 | | +2.430 |
| 38 | Ryouta Kamiyama (Neo) | JPN | 37.22 | | +2.480 |
| 39 | Tomoya Ishihara (Neo) | JPN | 37.38 | | +2.640 |
| 40 | Mahiro Hara (Neo) | JPN | 37.57 | | +2.830 |
| 41 | Keiryuu Yoneda (Neo) | JPN | 37.68 | | +2.940 |
| 42 | Iichiro Yui (Neo) | JPN | 37.88 | | +3.140 |
| 43 | Ryuunosuke Oomichi (A2) | JPN | 38.21 | | +3.470 |
| 44 | Haruki Gomi (A1) | JPN | 38.44 | | +3.700 |
| 45 | Shouta Hayashi (A1) | JPN | 38.84 | | +4.100 |
| 46 | Yamato Matsui (Neo) | JPN | 1:01.96 | | +27.220 |
| 47 | Kazuya Anbo (Neo) | JPN | 1:09.41 | | +34.670 |
| DNS | Taiyo Nonomura (A2) | JPN | DNS | | - |