| 1 | Miki Aro (Neo) | JPN | 38.81 | PB | - |
| 2 | Hina Onodera (Neo) | JPN | 39.35 | | +0.540 |
| 3 | Maria Victoria Rodriguez (Senior) | ARG | 39.45 | | +0.640 |
| 4 | Fumina Ishioka (A1) | JPN | 40.01 | | +1.200 |
| 5 | Victoria Goplen (Neo) | CAN | 40.07 | | +1.260 |
| 6 | Blair Cruikshank (Senior) | USA | 40.08 | | +1.270 |
| 7 | Towako Nagasaki (Senior) | JPN | 40.09 | SB | +1.280 |
| 8 | Anna Quinn (Senior) | USA | 40.41 | SB | +1.600 |
| 9 | Piper Yde (Neo) | USA | 40.61 | | +1.800 |
| 10 | Libby Williams (Neo) | USA | 40.92 | | +2.110 |
| 11 | Mei Takeda (B1) | JPN | 41.16 | PB | +2.350 |
| 12 | Ilsa Melin Sandvig Shobe (Senior) | USA | 41.36 | | +2.550 |
| 13 | Marley Soldan (A1) | USA | 41.39 | SB | +2.580 |
| 14 | Jenni Kukkonen (M35) | FIN | 41.42 | NRM | +2.610 |
| 15 | Maya Lueders (A2) | CAN | 41.47 | | +2.660 |
| 16 | Amy Acosta (Neo) | ECU | 41.72 | | +2.910 |
| 17 | Giorgia Birkeland (Neo) | USA | 41.89 | | +3.080 |
| 18 | Karen Lizeth Siatama Valencia (A2) | COL | 42.17 | | +3.360 |
| 19 | Natalia Valencia (Senior) | COL | 42.33 | | +3.520 |
| 20 | Elise Stave (B2) | USA | 42.54 | | +3.730 |
| 21 | Shruti Kotwal (M30) | IND | 42.63 | | +3.820 |
| 22 | Keeley Whitcomb (B2) | USA | 42.65 | PB | +3.840 |
| 23 | Elena Stave (C2) | USA | 43.14 | | +4.330 |
| 24 | Cynthia Jennings (Senior) | USA | 43.53 | | +4.720 |
| 25 | Evie Forrer (B1) | USA | 44.04 | | +5.230 |
| 26 | Aileen Oh (C1) | USA | 45.80 | | +6.990 |
| 27 | Audrey Han (B2) | USA | 46.60 | | +7.790 |
| 28 | Kassidy González Mcglauchlen (C2) | USA | 48.39 | | +9.580 |
| DQ | Alejandra Huertas (B2) | COL | DQ | | - |