| 1 | Sara van Leeuwen (A2) | NED | 42.38 | | - |
| 2 | Maud Groenen (B1) | NED | 42.99 | PB | +0.610 |
| 3 | Naomi Wessels (B1) | NED | 43.19 | PB | +0.810 |
| 4 | Amina Dillmann (B1) | NED | 43.60 | | +1.220 |
| 5 | Amanda van Vugt (C2) | NED | 43.64 | | +1.260 |
| 6 | Martha Trude Brehmer (C2) | SWE | 43.66 | PB | +1.280 |
| 7 | Melle Boeijen (A1) | NED | 43.75 | | +1.370 |
| 8 | Carlijn te Poele (C1) | NED | 43.82 | | +1.440 |
| 9 | Youssra Pals (B1) | NED | 44.56 | | +2.180 |
| 10 | Nynke Lankhaar (A1) | NED | 45.52 | SB | +3.140 |
| 11 | Carlijn Oude Vrielink (Neo) | NED | 45.59 | PB | +3.210 |
| 12 | Hiba Pals (C1) | NED | 46.48 | PB | +4.100 |
| 13 | Roos de Vrieze (B2) | NED | 46.70 | | +4.320 |
| 14 | Maartje van Dijck (C2) | NED | 47.10 | PB | +4.720 |
| 15 | Yasmine Pals (B1) | NED | 47.45 | | +5.070 |
| 16 | Tamar Wolters (C2) | NED | 47.62 | PB | +5.240 |
| 17 | Naomi Segers (D2) | NED | 48.74 | | +6.360 |
| 18 | Loïs Mijland (D1) | NED | 48.81 | PB | +6.430 |
| 19 | Bregje Nafzger (B1) | NED | 50.58 | PB | +8.200 |
| 20 | Jojanneke den Hartog (D1) | NED | 51.08 | | +8.700 |
| 21 | Floor Vogelaar (D1) | NED | 51.54 | PB | +9.160 |
| 22 | Lena Westra (D1) | NED | 52.43 | PB | +10.050 |
| 23 | Isabel Segers (C2) | NED | 53.10 | | +10.720 |
| 24 | Patty van Hoorn (M50) | NED | 53.50 | SB | +11.120 |
| 25 | Zoé van der Laar (C1) | NED | 54.02 | PB | +11.640 |
| 26 | Jip van Deijck (B1) | NED | 54.05 | PB | +11.670 |
| 27 | Michelle van Sonsbeeck (M55) | NED | 54.69 | SB | +12.310 |
| 28 | Neel Geurts (D1) | NED | 55.18 | PB | +12.800 |
| 29 | Senna Coenraad (D2) | NED | 55.33 | PB | +12.950 |
| 30 | Martine Margadant (M55) | NED | 55.88 | SB | +13.500 |
| 31 | Cato Gunneweg (D1) | NED | 57.94 | | +15.560 |
| 32 | Otje Bilderbeek (E2) | NED | 58.18 | PB | +15.800 |
| 33 | Giorgia Dingemans (E1) | NED | 58.32 | PB | +15.940 |
| 34 | Sophia Coenraad (D2) | NED | 58.43 | PB | +16.050 |
| 35 | Sammie Koreman (E2) | NED | 58.58 | PB | +16.200 |
| 36 | Amélie van den Berk (E2) | NED | 1:03.74 | | +21.360 |