| 1 | Anna Dijkers (B2) | NED | 43.36 | | - |
| 2 | Isabelle Hersbach (A1) | NED | 43.94 | | +0.580 |
| 3 | Sylvia Ammerlaan (Senior) | NED | 46.08 | SB | +2.720 |
| 4 | Jane Ravestein (Senior) | NED | 46.14 | SB | +2.780 |
| 5 | Sanne Knijnenburg (Neo) | NED | 46.95 | | +3.590 |
| 6 | Milou Zuiderwijk (A1) | NED | 47.45 | | +4.090 |
| 7 | Anna Eijsackers (C1) | NED | 47.47 | | +4.110 |
| 8 | Amber van der Meijden (D2) | NED | 47.66 | | +4.300 |
| 9 | Kiki van Schaik (C1) | NED | 47.96 | | +4.600 |
| 10 | Yiran van 'T Oosten (D1) | NED | 49.79 | | +6.430 |
| 11 | Caitlin Koenen (C1) | NED | 50.81 | | +7.450 |
| 12 | Marcella van de Weem (B1) | NED | 51.36 | SB | +8.000 |
| 13 | Hilde Dijkers (C1) | NED | 52.69 | SB | +9.330 |
| 14 | Maaike van Zijll (Senior) | NED | 52.74 | | +9.380 |
| 15 | Nienke Burger (D2) | NED | 54.96 | | +11.600 |
| 16 | Britney van der Steen (A2) | NED | 56.69 | SB | +13.330 |
| 17 | Elske van Raaphorst (Senior) | NED | 56.75 | SB | +13.390 |
| 18 | Stefanie Herrebout (C1) | NED | 56.77 | SB | +13.410 |
| 19 | Evelien Ras (D1) | NED | 57.21 | | +13.850 |
| 20 | Melissa Buijnink (Neo) | NED | 58.45 | SB | +15.090 |
| 21 | Veronica Veerkamp (M30) | NED | 59.40 | SB | +16.040 |
| 22 | Esmee van der Meer (D1) | NED | 59.58 | | +16.220 |
| 23 | Kirya Aldewereld (Neo) | NED | 1:03.21 | SB | +19.850 |
| 24 | Lenthe Jansen (D1) | NED | 1:03.55 | SB | +20.190 |
| 25 | Fleur Van Zeijl (D1) | NED | 1:06.46 | SB | +23.100 |
| 26 | Roos Weber (M50) | NED | 1:10.47 | SB | +27.110 |
| 27 | Jadyn Hazelhorst (D1) | NED | 1:15.61 | PB | +32.250 |
| 28 | Guusje Loncke (D1) | NED | 1:19.30 | | +35.940 |