| 1 | Tamar van der Zwaan (Neo) | NED | 45.03 | | - |
| 2 | Manouk van Emmerik (Senior) | NED | 45.11 | | +0.080 |
| 3 | Annet van der Stelt (C1) | NED | 45.38 | | +0.350 |
| 4 | Nadine van den Hoek (A2) | NED | 45.70 | | +0.670 |
| 5 | Nicky van Leeuwen (C2) | NED | 46.06 | | +1.030 |
| 6 | Saskia Rewijk (B2) | NED | 46.08 | PB | +1.050 |
| 7 | Ellen van Velzen (Neo) | NED | 46.20 | PB | +1.170 |
| 8 | Crissie de Jeu (B1) | NED | 47.29 | PB | +2.260 |
| 9 | Roos van der Zwaan (A1) | NED | 47.84 | | +2.810 |
| 10 | Esmee Visser (C1) | NED | 47.88 | | +2.850 |
| 11 | Anneloes Langeveld (Neo) | NED | 47.97 | | +2.940 |
| 12 | Deborah van der Stelt (C1) | NED | 48.82 | | +3.790 |
| 13 | Yumiko van Diest (D2) | NED | 49.46 | | +4.430 |
| 14 | Jamie Schelling (D1) | NED | 49.62 | | +4.590 |
| 15 | Moniek Langhout (A1) | NED | 50.14 | | +5.110 |
| 16 | Suzanne Kuijpers (C1) | NED | 51.98 | SB | +6.950 |
| 17 | Shanna Herweijer (B2) | NED | 52.05 | SB | +7.020 |
| 18 | Angela van Wijk (M40) | NED | 52.86 | | +7.830 |
| 19 | Steffi van der Meer (C1) | NED | 55.01 | | +9.980 |
| 20 | Helmy de Koning (C2) | NED | 55.06 | SB | +10.030 |
| 21 | Julia Visser (C1) | NED | 55.41 | | +10.380 |
| 22 | Shannon van Dam (C1) | NED | 55.85 | PB | +10.820 |
| 23 | Roëlle de Koning (D2) | NED | 55.91 | SB | +10.880 |
| 24 | Samantha Meyer (D2) | NED | 56.58 | | +11.550 |
| 25 | Wendy Straathof (B1) | NED | 56.96 | | +11.930 |
| 26 | Lilian Schoots (C1) | NED | 57.48 | | +12.450 |
| 27 | Roos-Anne van Rugge (D2) | NED | 58.66 | SB | +13.630 |
| 28 | Janine Vonk (C1) | NED | 59.52 | | +14.490 |
| 29 | Loena Visser (D1) | NED | 1:01.13 | | +16.100 |
| 30 | Alicia de Groot (C2) | NED | 1:03.10 | SB | +18.070 |
| 31 | Chantal van Bekkum (D2) | NED | 1:04.20 | SB | +19.170 |
| 32 | Donna Vermeulen (E1) | NED | 1:04.25 | SB | +19.220 |
| 33 | Marit Beukers (D2) | NED | 1:06.71 | SB | +21.680 |
| 34 | Martine Straathof (D2) | NED | 1:06.74 | SB | +21.710 |
| 35 | Willemijn Henrotte (D2) | NED | 1:10.20 | PB | +25.170 |
| 36 | Eveline van der Bie (C2) | NED | 1:11.92 | | +26.890 |
| 37 | Jacqueline Langeveld (M50) | NED | 1:12.59 | SB | +27.560 |
| 38 | Carla Engele (M45) | NED | 1:14.80 | SB | +29.770 |
| 39 | Alissa de Koning (E2) | NED | 1:15.82 | | +30.790 |
| 40 | Lindsay Pecht (F2) | NED | 1:16.81 | SB | +31.780 |
| 41 | Gea Straathof (M45) | NED | 1:21.71 | SB | +36.680 |
| 42 | Daphne Kompier (E2) | NED | 1:29.31 | PB | +44.280 |
| DQ | Marjolein van der Zwaard (E1) | NED | DQ | | - |