| 1 | Marjolein Ooms (C2) | NED | 44.26 | PB | - |
| 2 | Tessa Dijksman (Senior) | NED | 45.15 | SB | +0.890 |
| 3 | Maureen Hagen (A2) | NED | 45.26 | | +1.000 |
| 4 | Renske van der Veer (Neo) | NED | 45.29 | SB | +1.030 |
| 5 | Lianne van Assema (B1) | NED | 45.51 | PB | +1.250 |
| 6 | Melanie Ooms (D2) | NED | 46.01 | | +1.750 |
| 7 | Britt van Wijk (B2) | NED | 46.81 | | +2.550 |
| 8 | Noa Bierens (B2) | NED | 47.49 | PB | +3.230 |
| 9 | Fleur Kleijne (Senior) | NED | 47.68 | PB | +3.420 |
| 10 | Ciska de Ree (M35) | NED | 47.90 | | +3.640 |
| 11 | Iris Meerhoff (Senior) | NED | 49.06 | SB | +4.800 |
| 12 | Renske Bruinsma (Senior) | NED | 49.76 | PB | +5.500 |
| 13 | Valerie de Graaf (n/a) | NED | 55.81 | PB | +11.550 |
| 14 | Sonja Beemsterboer (M45) | NED | 1:00.28 | PB | +16.020 |
| Valerie Rinses (D2) | NED | 1:00.28 | PB | +16.020 |
| 16 | Sanne Neijzing (D2) | NED | 1:00.84 | | +16.580 |
| 17 | Layla Geschev (E2) | NED | 1:01.06 | | +16.800 |
| 18 | Anneli Eijkenaar (D2) | NED | 1:04.71 | PB | +20.450 |
| 19 | Anaïs van Broekhoven (n/a) | NED | 1:05.11 | PB | +20.850 |
| 20 | Tessel Ruiter (A1) | NED | 1:05.32 | SB | +21.060 |
| 21 | Yfke Fopma (E2) | NED | 1:10.21 | PB | +25.950 |
| 22 | Iris van der Pol (n/a) | NED | 1:11.28 | PB | +27.020 |
| DNS | Olivia Windrich Vega (n/a) | NED | DNS | | - |