| 1 | Ellen Barendregt (Senior) | NED | 46.21 | | - |
| 2 | Jolien van den Berg (Senior) | NED | 46.53 | | +0.320 |
| 3 | Emma de Fraiture (A2) | NED | 46.77 | | +0.560 |
| 4 | Esther van Leeuwen (Neo) | NED | 47.83 | | +1.620 |
| 5 | Frederieke Jansen (Neo) | NED | 47.92 | | +1.710 |
| 6 | Nienke Hinsenveld (Neo) | NED | 48.01 | | +1.800 |
| 7 | Malou van Slobbe (Neo) | NED | 48.47 | | +2.260 |
| 8 | Danique Ammerlaan (Neo) | NED | 48.59 | | +2.380 |
| 9 | Kim Temmink (Senior) | NED | 49.59 | | +3.380 |
| 10 | Minou Groenen (Neo) | NED | 50.81 | | +4.600 |
| 11 | Lianne van Os (Neo) | NED | 51.58 | | +5.370 |
| 12 | Lisa van Diepen (Neo) | NED | 52.38 | SB | +6.170 |
| 13 | Meike de Jong (Neo) | NED | 52.93 | | +6.720 |
| 14 | Annelotte van 't Land (Neo) | NED | 55.68 | | +9.470 |
| 15 | Iris Klok (Neo) | NED | 55.85 | | +9.640 |
| 16 | Emma van Leersum (Senior) | NED | 1:00.21 | | +14.000 |
| 17 | Fenna Visser (A2) | NED | 1:01.14 | | +14.930 |
| 18 | Anoeska Bos (Neo) | NED | 1:04.15 | | +17.940 |
| 19 | Mariska van den Hove (Neo) | NED | 1:06.84 | | +20.630 |
| 20 | Sara van Laar (Neo) | NED | 1:15.02 | | +28.810 |
| 21 | Lisanne Nendels (Senior) | NED | 1:17.65 | PB | +31.440 |
| 22 | Stephanie Kruiper (Neo) | NED | 1:26.62 | SB | +40.410 |
| 23 | Kim Custers (Neo) | NED | 1:34.01 | SB | +47.800 |
| DNS | Janneke Hulsen (Neo) | NED | DNS | | - |