| 1 | Gina Haisma (B2) | NED | 45.07 | | - |
| 2 | Anjo de Jong (B2) | NED | 45.13 | | +0.060 |
| 3 | Jolijn de Vries (Senior) | NED | 45.22 | SB | +0.150 |
| 4 | Jilldau Tjalma (B2) | NED | 45.28 | PB | +0.210 |
| 5 | Sanne van der Schaar (B2) | NED | 45.38 | | +0.310 |
| 6 | Rianne Jorritsma (C1) | NED | 45.42 | | +0.350 |
| 7 | Japke Janneke Sybesma (A2) | NED | 45.48 | SB | +0.410 |
| 8 | Jolien Scholtens (B2) | NED | 45.68 | | +0.610 |
| 9 | Roza Kooystra (C1) | NED | 45.85 | SB | +0.780 |
| 10 | Carin Ketellapper (Senior) | NED | 46.16 | | +1.090 |
| 11 | Annemarie Kuiper (C2) | NED | 46.37 | | +1.300 |
| 12 | Iris Scholtens (C1) | NED | 46.40 | | +1.330 |
| 13 | Judith Zijlstra (Neo) | NED | 47.01 | PB | +1.940 |
| 14 | Lisa van Eek (C2) | NED | 47.06 | | +1.990 |
| 15 | Corina Dijkstra (C1) | NED | 47.15 | | +2.080 |
| 16 | Velda Tjalma (C2) | NED | 47.90 | | +2.830 |
| 17 | Angela Dijkstra (B1) | NED | 48.07 | | +3.000 |
| 18 | Janneke Hospes (M30) | NED | 48.24 | | +3.170 |
| 19 | Brecht Kalsbeek (B1) | NED | 48.48 | | +3.410 |
| 20 | Jinke Miriam van Keulen (C2) | NED | 48.89 | | +3.820 |
| Ulrike Weerman (C1) | NED | 48.89 | SB | +3.820 |
| 22 | Chantal Oosterkamp (C1) | NED | 48.91 | | +3.840 |
| 23 | Simone Postma (C2) | NED | 48.94 | | +3.870 |
| 24 | Joyce Bakker (C1) | NED | 49.35 | | +4.280 |
| 25 | Brechtje-Rinkje de Boer (A1) | NED | 49.89 | PB | +4.820 |
| 26 | Doetie Vos-van der Meulen (M45) | NED | 51.48 | | +6.410 |
| 27 | Esmee Sierdsma (C1) | NED | 51.87 | | +6.800 |
| 28 | Ilse Vos (B2) | NED | 52.87 | SB | +7.800 |
| 29 | Nienke Stoker (B1) | NED | 55.90 | | +10.830 |
| 30 | An Bennema (B1) | NED | 58.43 | SB | +13.360 |
| 31 | Wiep Folkerts (M60) | NED | 1:08.41 | | +23.340 |