| 1 | Kimi Goetz (Senior) | USA | 38.40 | | - |
| 2 | Chrysta Rands-Evans (Senior) | USA | 39.86 | | +1.460 |
| 3 | Greta Myers (A2) | USA | 40.34 | | +1.940 |
| 4 | Anna Quinn (Neo) | USA | 41.36 | | +2.960 |
| 5 | Laura Michelle Rodriguez (B2) | COL | 41.94 | | +3.540 |
| 6 | Lindsey Woodbury (Neo) | USA | 42.13 | SB | +3.730 |
| Libby Williams (A1) | USA | 42.13 | | +3.730 |
| 8 | Thalia Staehle (A2) | USA | 42.51 | | +4.110 |
| 9 | Natalia Valencia (Senior) | COL | 42.67 | | +4.270 |
| 10 | Shruti Kotwal (M30) | IND | 42.99 | | +4.590 |
| 11 | Larissa Paes (Senior) | BRA | 43.32 | | +4.920 |
| 12 | Marley Soldan (B1) | USA | 43.83 | | +5.430 |
| 13 | Rebecca Simmons (M30) | USA | 44.34 | | +5.940 |
| 14 | Isabela Vargas Rodriguez (B1) | COL | 44.98 | | +6.580 |
| 15 | Ella Teeples (C1) | USA | 46.28 | | +7.880 |
| 16 | Karen Lizeth Siatama Valencia (B2) | COL | 46.48 | | +8.080 |
| 17 | Leah Choi (C2) | USA | 47.58 | | +9.180 |
| 18 | Audrey Han (C2) | USA | 49.04 | | +10.640 |
| 19 | Kaitlyn Lee (C1) | USA | 49.56 | | +11.160 |
| 20 | Finnley D'Card (C1) | USA | 50.35 | | +11.950 |
| 21 | Juliette Song (D1) | USA | 52.38 | | +13.980 |
| 22 | Scarelette Gramlich Thatcher (D1) | USA | 53.20 | | +14.800 |
| 23 | Nihan Tatli (n/a) | USA | 1:02.29 | PB | +23.890 |
| 24 | Atira Meacham (F2) | USA | 1:09.25 | | +30.850 |