| 1 | Christine Aaftink (Senior) | NED | 41.04 | | - |
| 2 | Sandra Voetelink (Neo) | NED | 41.23 | | +0.190 |
| 3 | Anita Loorbach (Senior) | NED | 41.52 | | +0.480 |
| 4 | Marieke Stam (Senior) | NED | 41.71 | SB | +0.670 |
| 5 | Herma Emmens-Meijer (Neo) | NED | 41.83 | | +0.790 |
| 6 | Sandra Zwolle (Neo) | NED | 42.40 | SB | +1.360 |
| 7 | Renske Vellinga (B2) | NED | 42.63 | NRJ | +1.590 |
| 8 | Annamarie Thomas (Neo) | NED | 43.16 | | +2.120 |
| Anja Bollaart (Senior) | NED | 43.16 | | +2.120 |
| 10 | Andrea Nuyt (B2) | NED | 43.47 | SB | +2.430 |
| Tineke Hulzebosch (Senior) | NED | 43.47 | SB | +2.430 |
| Gonnie Brunia (Neo) | NED | 43.47 | SB | +2.430 |
| 13 | Barbara de Loor (A1) | NED | 43.53 | | +2.490 |
| Janine Koudenburg (Neo) | NED | 43.53 | | +2.490 |
| 15 | Manuela Ossendrijver (Neo) | NED | 43.85 | | +2.810 |
| 16 | Carla Zijlstra (Neo) | NED | 44.49 | | +3.450 |