| 1 | Janine Smit (Senior) | NED | 38.80 | SB | - |
| 2 | Floor van den Brandt (Senior) | NED | 39.02 | | +0.220 |
| 3 | Letitia de Jong (Senior) | NED | 39.20 | | +0.400 |
| 4 | Danouk Bannink (Neo) | NED | 39.45 | SB | +0.650 |
| 5 | Fabienne Winkel (Neo) | NED | 40.47 | PB | +1.670 |
| 6 | Nille Martha Norhaug Faleide (Senior) | NOR | 40.54 | | +1.740 |
| 7 | Monica Tuijp (Neo) | NED | 40.78 | SB | +1.980 |
| 8 | Jenni Kukkonen (Senior) | FIN | 41.41 | | +2.610 |
| 9 | Julia Noordenbos (A2) | NED | 41.58 | | +2.780 |
| 10 | Tessa Sintenie (Neo) | NED | 41.87 | | +3.070 |
| 11 | Katja Lutz (Senior) | SUI | 42.58 | | +3.780 |
| 12 | Miriam Kapischke (A1) | GER | 43.21 | | +4.410 |
| 13 | Saskia Püllen (C2) | GER | 43.75 | SB | +4.950 |
| 14 | Steffi Rothenfußer (B1) | GER | 44.29 | | +5.490 |
| 15 | Tamara Groot (Senior) | NED | 44.62 | | +5.820 |
| 16 | Iris Smokers (Neo) | NED | 45.05 | SB | +6.250 |
| 17 | Jip van Elst (A2) | NED | 46.60 | SB | +7.800 |
| 18 | Aukje Zijlstra (Neo) | NED | 46.62 | SB | +7.820 |
| 19 | Annika Letteboer (D2) | GER | 46.78 | | +7.980 |
| 20 | Maira Jasch (D1) | GER | 46.85 | | +8.050 |
| 21 | Merel van Toorenburg (Neo) | NED | 47.37 | | +8.570 |
| 22 | Anke Weerts (Senior) | NED | 47.53 | SB | +8.730 |
| 23 | Julia Bachl (C1) | GER | 48.24 | | +9.440 |
| 24 | Marie Motschmann (C2) | GER | 48.28 | | +9.480 |
| 25 | Lena Arens (B1) | GER | 48.46 | SB | +9.660 |
| 26 | Ashley Völker (D1) | GER | 48.61 | | +9.810 |
| 27 | Clea Zimmermann (C2) | GER | 48.64 | | +9.840 |
| 28 | Leonie Mayrhofer (D2) | GER | 48.74 | | +9.940 |
| 29 | Saskia van der Hoeven (A1) | NED | 49.14 | PB | +10.340 |
| 30 | Geke Kloosterziel (Neo) | NED | 49.25 | | +10.450 |
| 31 | Sanne Veenstra (Neo) | NED | 49.60 | SB | +10.800 |
| 32 | Nina Mayrhofer (D2) | GER | 49.80 | | +11.000 |
| 33 | Iris Zahn (Neo) | NED | 50.51 | | +11.710 |
| 34 | Sandy van der Pols (B1) | NED | 51.07 | PB | +12.270 |
| 35 | Stina Letteboer (E2) | GER | 51.10 | | +12.300 |
| 36 | Isabel Kraus (D1) | GER | 51.63 | | +12.830 |
| 37 | Sophie Patz (E2) | GER | 51.74 | | +12.940 |
| 38 | Renee van Boekel (Neo) | NED | 52.94 | | +14.140 |
| 39 | Julia Geutner (E2) | GER | 53.08 | | +14.280 |
| 40 | Laura Geurts (Senior) | NED | 53.28 | | +14.480 |
| 41 | Rachelle Maassen (Neo) | NED | 53.31 | SB | +14.510 |
| 42 | Eliska Fackenbergova (E2) | CZE | 54.59 | | +15.790 |
| 43 | Barbora Slámová (F2) | CZE | 55.34 | SB | +16.540 |
| 44 | Maite van der Knaap (Neo) | NED | 55.76 | SB | +16.960 |
| 45 | Robin van der Steen (Neo) | NED | 57.04 | | +18.240 |
| 46 | Monika Macháčková (E1) | CZE | 57.14 | | +18.340 |
| 47 | Maxime van Riel (n/a) | NED | 57.74 | SB | +18.940 |
| 48 | Eliska Hunackova (E1) | CZE | 58.57 | | +19.770 |
| 49 | Adéla Krejčová (F2) | CZE | 59.59 | | +20.790 |
| 50 | Katja Heidweiller (Neo) | NED | 1:00.80 | | +22.000 |
| 51 | Kristýna Němcová (E1) | CZE | 1:02.36 | | +23.560 |
| 52 | Evi de Ruiter (E1) | NED | 1:04.74 | SB | +25.940 |
| 53 | Lydia Overtoom (Senior) | NED | 1:05.12 | | +26.320 |
| 54 | Richtje Taal (M65) | NED | 1:10.49 | | +31.690 |
| 55 | Amálie Doláková (F1) | CZE | 1:21.22 | | +42.420 |