| 1 | Milou Butter (Neo) | NED | 45.60 | SB | - |
| Maureen Hagen (A2) | NED | 45.60 | SB | - |
| 3 | Anouk Könst (A2) | NED | 45.61 | | +0.010 |
| 4 | Lara Dingjan (Neo) | NED | 45.62 | | +0.020 |
| 5 | Maartje Heine (Senior) | NED | 45.98 | SB | +0.380 |
| 6 | Tessa Dijksman (Senior) | NED | 46.38 | SB | +0.780 |
| Stefanie Koot (B2) | NED | 46.38 | | +0.780 |
| 8 | Zhara Möllers (A1) | NED | 46.47 | | +0.870 |
| 9 | Renske van der Veer (Neo) | NED | 46.94 | SB | +1.340 |
| 10 | Lianne van Assema (B1) | NED | 47.24 | SB | +1.640 |
| 11 | Britt van Wijk (B2) | NED | 47.47 | SB | +1.870 |
| 12 | Merit de Bruin (A2) | NED | 47.54 | SB | +1.940 |
| 13 | Kersty Heeremans (Neo) | NED | 48.10 | | +2.500 |
| 14 | Bente Adema (B1) | NED | 48.56 | SB | +2.960 |
| 15 | Noa Bierens (B2) | NED | 48.80 | SB | +3.200 |
| 16 | Anouk Rodenburg (B2) | NED | 49.00 | SB | +3.400 |
| 17 | Annette Laarhoven (A1) | NED | 49.12 | | +3.520 |
| 18 | Fleur Kleijne (Senior) | NED | 49.71 | SB | +4.110 |
| 19 | Amy Valentijn (B2) | NED | 49.73 | | +4.130 |
| 20 | Sandra Bijlert-Burgerhout (M45) | NED | 49.90 | SB | +4.300 |
| 21 | Charline Coates-Kwadrin (A1) | GBR | 50.05 | | +4.450 |
| 22 | Emma Hartveld (Neo) | NED | 50.23 | SB | +4.630 |
| 23 | Imke van Dongen (B1) | NED | 50.44 | SB | +4.840 |
| 24 | Eline Schmutzer (B1) | NED | 50.48 | SB | +4.880 |
| 25 | Sylvie van den Heuvel (Neo) | NED | 50.49 | | +4.890 |
| 26 | Sanne Ganzinga (A2) | NED | 50.88 | SB | +5.280 |
| 27 | Anne Lubbers (B2) | NED | 51.50 | SB | +5.900 |
| 28 | Caroline Broekhuizen (A2) | NED | 52.00 | SB | +6.400 |
| 29 | Wieteke Huiberts (Neo) | NED | 52.89 | SB | +7.290 |
| 30 | Lisanne Hartveld (B1) | NED | 53.48 | | +7.880 |
| 31 | Franca Baars (B1) | NED | 53.71 | | +8.110 |
| 32 | Pien Lodder (Neo) | NED | 53.93 | SB | +8.330 |
| 33 | Mirthe Visser (B2) | NED | 57.37 | SB | +11.770 |
| 34 | Judith van Hesselingen (M55) | NED | 57.88 | SB | +12.280 |
| 35 | Marijne Spijksma (B1) | NED | 58.64 | | +13.040 |
| 36 | Zoe Verhagen (B1) | NED | 1:01.46 | | +15.860 |
| 37 | Ramona van Leeuwen-van Noort (M55) | NED | 1:01.73 | SB | +16.130 |