| 1 | Esther Withagen (A2) | NED | 41.95 | PB | - |
| 2 | Marjolein van der Steen (Senior) | NED | 42.08 | SB | +0.130 |
| 3 | Silke Kabel (B1) | NED | 42.50 | PB | +0.550 |
| 4 | Liotte Smits (Neo) | NED | 42.63 | | +0.680 |
| 5 | Marloes Westerhof (M35) | NED | 42.83 | | +0.880 |
| 6 | Daphne van Kooten (C2) | NED | 43.29 | SB | +1.340 |
| 7 | Emma van Beek (C1) | NED | 43.36 | SB | +1.410 |
| 8 | Talis Renes (B2) | NED | 43.43 | | +1.480 |
| 9 | Luna van Polen (A1) | NED | 43.98 | | +2.030 |
| 10 | Nienke Meesters (C1) | NED | 43.99 | | +2.040 |
| 11 | Vita van Deuren (C2) | NED | 44.42 | PB | +2.470 |
| 12 | Maureen van Seumeren (C1) | NED | 44.57 | SB | +2.620 |
| 13 | Diede Wallet (Neo) | NED | 44.67 | PB | +2.720 |
| 14 | Janneke Hennipman (Senior) | NED | 45.02 | SB | +3.070 |
| 15 | Anniek Heethaar (B1) | NED | 45.11 | PB | +3.160 |
| 16 | Renske van Winkoop (D1) | NED | 45.22 | | +3.270 |
| 17 | Janne Kraamer (Neo) | NED | 45.25 | | +3.300 |
| 18 | Norah Smits (Neo) | NED | 45.45 | PB | +3.500 |
| 19 | Tinne Bolsius (D2) | NED | 46.05 | SB | +4.100 |
| 20 | Kim Leliveld (M30) | NED | 46.23 | | +4.280 |
| 21 | Miranda van den Akker (C2) | NED | 46.27 | PB | +4.320 |
| 22 | Nina Heethaar (C1) | NED | 46.28 | PB | +4.330 |
| 23 | Jade Repko (A2) | NED | 46.33 | | +4.380 |
| 24 | Maartje Onderdelinden (C2) | NED | 46.37 | PB | +4.420 |
| 25 | Ella Boonstra (C2) | NED | 46.70 | SB | +4.750 |
| 26 | Josephine Dekkers (C2) | NED | 47.09 | SB | +5.140 |
| 27 | Anna van der Stok (M50) | NED | 47.19 | PB | +5.240 |
| 28 | Nelleke van de Wetering (M40) | NED | 47.31 | SB | +5.360 |
| 29 | Juul van der Vliet (D2) | NED | 47.33 | PB | +5.380 |
| 30 | Manon van de Vliert-Scholte (M50) | NED | 47.36 | SB | +5.410 |
| 31 | Ilse Kolmer (C1) | NED | 47.49 | PB | +5.540 |
| 32 | Sarah Wijgergangs (C1) | NED | 48.18 | PB | +6.230 |
| 33 | Linde Rijnders (Neo) | NED | 48.45 | PB | +6.500 |
| 34 | Senne Eggenkamp (D2) | NED | 48.47 | | +6.520 |
| 35 | Jennifer Groeneveld (D2) | NED | 48.77 | SB | +6.820 |
| 36 | Maud Heersink (D2) | NED | 49.02 | | +7.070 |
| 37 | Bente van Dasselaar (C1) | NED | 49.06 | PB | +7.110 |
| 38 | Dara van Hees (D2) | NED | 49.08 | SB | +7.130 |
| 39 | Ella van der Sijde (A1) | NED | 49.10 | SB | +7.150 |
| 40 | Pia Rudiger (B1) | NED | 49.28 | SB | +7.330 |
| 41 | Linda Eggenkamp (M50) | NED | 49.63 | | +7.680 |
| 42 | Nanne Kabel (C2) | NED | 50.12 | SB | +8.170 |
| 43 | Julia Onderdelinden (D2) | NED | 50.58 | SB | +8.630 |
| 44 | Noor Schaap (D1) | NED | 50.66 | SB | +8.710 |
| 45 | Esther Rüdiger (C2) | NED | 50.67 | SB | +8.720 |
| 46 | Tessa van der Sande (C1) | NED | 50.72 | | +8.770 |
| 47 | Mirthe Snoek (D1) | NED | 51.08 | | +9.130 |
| 48 | Tess van Deijin (A1) | NED | 51.35 | PB | +9.400 |
| 49 | Mara Putman (D1) | NED | 52.84 | SB | +10.890 |
| 50 | Mia Brons (D1) | NED | 53.03 | SB | +11.080 |
| 51 | Fiene Rits (D1) | NED | 53.41 | SB | +11.460 |
| 52 | Sofie van Veen (C1) | NED | 53.80 | SB | +11.850 |
| 53 | Maartje Boonzaaijer (C2) | NED | 54.29 | PB | +12.340 |
| 54 | Linn Kolkman (D2) | NED | 54.78 | SB | +12.830 |
| 55 | Marleine Veldhuizen (D1) | NED | 55.46 | PB | +13.510 |
| 56 | Hannah Jacobs (E2) | NED | 57.22 | SB | +15.270 |
| 57 | Anniek Eijpe (D1) | NED | 57.25 | SB | +15.300 |
| 58 | Milou van Dam (E1) | NED | 58.67 | SB | +16.720 |
| 59 | Isa Groenestein (E2) | NED | 58.98 | PB | +17.030 |
| 60 | Meike Voorneveld (D1) | NED | 59.13 | PB | +17.180 |
| 61 | Jo-Ann Nabuurs (E2) | NED | 1:02.82 | SB | +20.870 |
| 62 | Vieve Alink (D1) | NED | 1:05.08 | | +23.130 |
| 63 | Nienke Snijder (E2) | NED | 1:07.10 | PB | +25.150 |
| DQ | Femke Horstman (E2) | NED | DQ | | - |