| 1 | Elina Risku (Senior) | FIN | 39.01 | | - |
| 2 | Marrit Leenstra (Senior) | NED | 39.20 | | +0.190 |
| 3 | Floor van den Brandt (Senior) | NED | 39.33 | | +0.320 |
| 4 | Anice Das (Senior) | NED | 39.39 | | +0.380 |
| 5 | Gabriele Hirschbichler (Senior) | GER | 39.53 | | +0.520 |
| 6 | Mayon Kuipers (Senior) | NED | 39.77 | | +0.760 |
| 7 | Manon Kamminga (Senior) | NED | 39.85 | SB | +0.840 |
| 8 | Xin.92 Zhao (Neo) | CHN | 39.87 | | +0.860 |
| 9 | Rosa Pater (Senior) | NED | 39.88 | | +0.870 |
| 10 | Marije Joling (Senior) | NED | 39.94 | SB | +0.930 |
| 11 | Sanneke de Neeling (Neo) | NED | 39.99 | | +0.980 |
| 12 | Annette Gerritsen (Senior) | NED | 40.02 | | +1.010 |
| 13 | Moniek Klijnstra (Neo) | NED | 40.04 | | +1.030 |
| 14 | Yvonne Daldossi (Senior) | ITA | 40.13 | | +1.120 |
| 15 | Isabelle van Elst (A1) | NED | 40.20 | | +1.190 |
| 16 | Roxanne van Hemert (Senior) | NED | 40.27 | | +1.260 |
| 17 | Ksenija Sadovskaja (Senior) | BLR | 40.29 | | +1.280 |
| 18 | Lotte van Beek (Senior) | NED | 40.37 | | +1.360 |
| 19 | Roxanne Dufter (Senior) | GER | 40.39 | | +1.380 |
| 20 | Natalia Czerwonka (Senior) | POL | 40.44 | | +1.430 |
| 21 | Linda de Vries (Senior) | NED | 40.45 | | +1.440 |
| 22 | Aveline Hylkema (Neo) | NED | 40.52 | | +1.510 |
| 23 | Sanne van der Schaar (Neo) | NED | 40.60 | | +1.590 |
| 24 | Elisa Dul (B2) | NED | 40.74 | SB | +1.730 |
| 25 | Annemarie Boer (Neo) | NED | 40.76 | | +1.750 |
| 26 | Julia Berentschot (Neo) | NED | 40.78 | SB | +1.770 |
| 27 | Lina Miedema (A1) | NED | 40.81 | | +1.800 |
| 28 | Tatjana Mikhajlova (Senior) | BLR | 40.83 | | +1.820 |
| 29 | Bianca Roosenboom (Senior) | NED | 40.93 | | +1.920 |
| 30 | Francesca Lollobrigida (Senior) | ITA | 40.98 | | +1.970 |
| 31 | Jorien Voorhuis (Senior) | NED | 41.03 | | +2.020 |
| 32 | Fabienne Winkel (Neo) | NED | 41.05 | | +2.040 |
| 33 | Marina Zueva (Senior) | BLR | 41.12 | | +2.110 |
| 34 | Manouk van Tol (Senior) | NED | 41.16 | | +2.150 |
| 35 | Camilla Lund (Neo) | NOR | 41.32 | PB | +2.310 |
| 36 | Tessa van der Neut (Neo) | NED | 41.36 | | +2.350 |
| 37 | Diane Valkenburg (Senior) | NED | 41.39 | | +2.380 |
| 38 | Katarzyna Wozniak (Senior) | POL | 41.41 | | +2.400 |
| 39 | Steffi Wubben (Neo) | NED | 41.43 | | +2.420 |
| 40 | Eva-Sterre Bording (Neo) | NED | 41.51 | SB | +2.500 |
| Milena Syrnikova (A1) | RUS | 41.51 | | +2.500 |
| 42 | Marit Steunenberg (A1) | NED | 41.60 | | +2.590 |
| 43 | Willemijn Cnossen (Neo) | NED | 41.61 | | +2.600 |
| 44 | Angelika Fudalej (Neo) | POL | 41.70 | SB | +2.690 |
| 45 | Reina Anema (Neo) | NED | 41.73 | | +2.720 |
| 46 | Tessa Sintenie (Neo) | NED | 41.78 | | +2.770 |
| 47 | Miranda Dekker (Senior) | NED | 41.81 | | +2.800 |
| 48 | Rosanne Fischer (A2) | NED | 41.82 | | +2.810 |
| 49 | Gloria Malfatti (A2) | ITA | 41.83 | | +2.820 |
| 50 | Demi van Benthem (A1) | NED | 41.87 | | +2.860 |
| 51 | Edel Jasmijn Bording (A2) | NED | 41.89 | | +2.880 |
| 52 | Freya Reitsma (A1) | NED | 41.90 | | +2.890 |
| 53 | Deborah Grisenti (A1) | ITA | 41.91 | | +2.900 |
| 54 | Tjitske Wassenaar (Neo) | NED | 41.94 | SB | +2.930 |
| 55 | Ane By Farstad (B1) | NOR | 42.11 | | +3.100 |
| 56 | Linda Koomen (Neo) | NED | 42.16 | | +3.150 |
| 57 | Linda Bortolotti (Neo) | ITA | 42.19 | | +3.180 |
| 58 | Aleksandra Goss (Senior) | POL | 42.21 | | +3.200 |
| 59 | Carmen Dam (Neo) | NED | 42.22 | | +3.210 |
| 60 | Melinda Groot (Neo) | NED | 42.26 | | +3.250 |
| 61 | Yauheniya Varabyova (A1) | BLR | 42.27 | | +3.260 |
| 62 | Paulien Westerhof (Senior) | NED | 42.38 | | +3.370 |
| 63 | Kelly Eijsink (Neo) | NED | 42.40 | | +3.390 |
| 64 | Johanna Östlund (Senior) | SWE | 42.51 | | +3.500 |
| 65 | Esmee Visser (Neo) | NED | 42.53 | | +3.520 |
| 66 | Noemi Bonazza (A1) | ITA | 42.54 | | +3.530 |
| 67 | Kelly Schouten (Neo) | NED | 42.58 | PB | +3.570 |
| 68 | Iza Stekelenburg (A2) | NED | 42.70 | | +3.690 |
| 69 | Urszula Włodarczyk (Neo) | POL | 42.72 | | +3.710 |
| 70 | Magdalena Czyszczoń (Neo) | POL | 42.81 | SB | +3.800 |
| 71 | Miina Peltonen (Neo) | FIN | 42.84 | | +3.830 |
| 72 | Chiara Cristelli (A1) | ITA | 42.85 | | +3.840 |
| 73 | Marija Minibajeva (B1) | RUS | 43.17 | | +4.160 |
| 74 | Merel Brunsmann (A2) | NED | 43.20 | | +4.190 |
| 75 | Milana Kostenko (B1) | RUS | 43.21 | | +4.200 |
| Kristina Vottsjal (A1) | RUS | 43.21 | | +4.200 |
| 77 | Anna Mjøen Holstad (A1) | NOR | 44.22 | | +5.210 |
| 78 | Ellia Smeding (A1) | GBR | 44.27 | | +5.260 |
| 79 | Susanna Lehtiniemi (Neo) | FIN | 44.41 | SB | +5.400 |
| 80 | Sophie Webb (Senior) | USA | 44.86 | | +5.850 |
| 81 | Veera Sarkanen (Neo) | FIN | 44.97 | | +5.960 |
| 82 | Ingeborg Winther Storler (B2) | NOR | 46.21 | | +7.200 |
| 83 | Lian van Dongen (M50) | NED | 46.42 | | +7.410 |
| 84 | Severine Biermann (C2) | SUI | 49.57 | | +10.560 |
| DQ | Natasja Roest (Neo) | NED | DQ | | - |