| 1 | Kaja Ziomek-Nogal (Senior) | POL | 38.66 | SB | - |
| 2 | Karolina Bosiek (Senior) | POL | 38.86 | | +0.200 |
| 3 | Martyna Baran (Senior) | POL | 39.27 | | +0.610 |
| 4 | Iga Wojtasik (Senior) | POL | 39.39 | | +0.730 |
| 5 | Serena Pergher (Neo) | ITA | 39.40 | SB | +0.740 |
| 6 | Natalia Jabrzyk (Senior) | POL | 39.50 | PB | +0.840 |
| 7 | Wiktoria Dąbrowska (A1) | POL | 39.85 | SB | +1.190 |
| 8 | Olga Piotrowska (Senior) | POL | 39.86 | PB | +1.200 |
| 9 | Francesca Lollobrigida (Senior) | ITA | 40.12 | SB | +1.460 |
| 10 | Natalia Czerwonka (Senior) | POL | 40.21 | | +1.550 |
| 11 | Zofia Braun (A1) | POL | 40.55 | SB | +1.890 |
| 12 | Vera Güntert (Senior) | SUI | 40.58 | SB | +1.920 |
| 13 | Julia Grzywinska (A1) | POL | 40.79 | PB | +2.130 |
| 14 | Maybritt Vigl (Neo) | ITA | 40.91 | SB | +2.250 |
| 15 | Laura Peveri (Neo) | ITA | 41.00 | | +2.340 |
| 16 | Iga Smejda (A2) | POL | 41.23 | SB | +2.570 |
| 17 | Veronika Antosova (Senior) | CZE | 41.54 | SB | +2.880 |
| 18 | Victoria Szewczyk (B2) | POL | 41.65 | SB | +2.990 |
| 19 | Lataesha Narain (Neo) | NED | 41.88 | SB | +3.220 |
| 20 | Amelia Boos (B2) | POL | 41.97 | SB | +3.310 |
| 21 | Mathilde Pédronno (Senior) | FRA | 42.14 | NR | +3.480 |
| 22 | Zofia Marszałek (C2) | POL | 42.26 | SB | +3.600 |
| 23 | Liwia Kubin (Neo) | POL | 42.52 | | +3.860 |
| 24 | Nadia Machała (C2) | POL | 42.83 | PB | +4.170 |
| 25 | Villiina Suomalainen (Neo) | FIN | 42.90 | SB | +4.240 |
| 26 | Lucie Korvasova (Neo) | CZE | 43.13 | SB | +4.470 |
| 27 | Oliwia Wrońska (B2) | POL | 43.22 | SB | +4.560 |
| 28 | Katerina Macháckova (Neo) | CZE | 44.00 | SB | +5.340 |
| 29 | Patricia Johannessen (Neo) | NOR | 44.53 | SB | +5.870 |
| 30 | Sara Hluskova (B2) | CZE | 44.81 | PB | +6.150 |
| 31 | Alessandra J. Mazzetti (B2) | NOR | 44.88 | PB | +6.220 |
| 32 | Jesika Forstova (A2) | CZE | 44.99 | SB | +6.330 |
| 33 | Sofia Gerber Della Pietra (B1) | AUT | 45.04 | PB | +6.380 |
| 34 | Monika Macháckova (A1) | CZE | 45.72 | SB | +7.060 |
| 35 | Małgorzata Machnicka (C1) | POL | 46.29 | SB | +7.630 |
| 36 | Manon Fraboulet (Neo) | FRA | 48.28 | PB | +9.620 |
| 37 | Kateřina Vystrcilova (A1) | CZE | 50.39 | PB | +11.730 |
| DQ | Andżelika Wójcik (Senior) | POL | DQ | | - |