| 1 | Erin Jackson (Senior) | USA | 37.50 | SB | - |
| 2 | Brittany Bowe (Senior) | USA | 37.89 | SB | +0.390 |
| 3 | Vanessa Herzog (Senior) | AUT | 38.20 | | +0.700 |
| 4 | Sofia Thorup (Senior) | DEN | 38.75 | NR | +1.250 |
| 5 | McKenzie Browne (Senior) | USA | 39.21 | | +1.710 |
| 6 | Greta Myers (Neo) | USA | 39.62 | | +2.120 |
| 7 | Libby Williams (Neo) | USA | 41.34 | | +3.840 |
| 8 | Ilsa Melin Sandvig Shobe (Senior) | USA | 41.70 | | +4.200 |
| 9 | Emma de Bock (B1) | USA | 42.13 | | +4.630 |
| 10 | Natalia Valencia (Senior) | COL | 42.80 | | +5.300 |
| 11 | Larissa Paes (Senior) | BRA | 42.83 | SB | +5.330 |
| 12 | Shruti Kotwal (M30) | IND | 43.15 | | +5.650 |
| 13 | Karen Lizeth Siatama Valencia (A2) | COL | 43.32 | | +5.820 |
| 14 | Rebecca Simmons (M35) | USA | 44.35 | | +6.850 |
| 15 | Ella Teeples (B1) | USA | 44.64 | | +7.140 |
| 16 | Natascha Lindenskov (Neo) | DEN | 44.70 | | +7.200 |
| 17 | Amy Acosta (Neo) | ECU | 45.06 | | +7.560 |