| 1 | Sofia Thorup (Senior) | DEN | 38.30 | NR | - |
| 2 | Chrysta Rands-Evans (Senior) | USA | 38.75 | SB | +0.450 |
| 3 | McKenzie Browne (Senior) | USA | 39.51 | | +1.210 |
| 4 | Greta Myers (Neo) | USA | 39.55 | SB | +1.250 |
| 5 | Sarah Warren (Senior) | USA | 39.75 | SB | +1.450 |
| 6 | Maria Victoria Rodriguez (Senior) | ARG | 39.80 | SB | +1.500 |
| 7 | Libby Williams (Neo) | USA | 41.46 | | +3.160 |
| 8 | Emma de Bock (B1) | USA | 41.58 | | +3.280 |
| 9 | Natalia Valencia (Senior) | COL | 42.47 | | +4.170 |
| 10 | Isabela Vargas Rodriguez (A1) | COL | 42.77 | SB | +4.470 |
| 11 | Amy Acosta (Neo) | ECU | 42.90 | NR | +4.600 |
| 12 | Karen Siatama (A2) | COL | 43.05 | | +4.750 |
| 13 | Shruti Kotwal (M30) | IND | 43.19 | | +4.890 |
| DQ | Cynthia Jennings (Senior) | USA | DQ | | - |