| 1 | Andżelika Wójcik (Neo) | POL | 39.80 | SB | - |
| 2 | Yu-Ting Huang (Senior) | TPE | 40.38 | NR | +0.580 |
| 3 | Linda Bortolotti (Neo) | ITA | 40.62 | SB | +0.820 |
| 4 | Myron Koops (Neo) | NED | 41.29 | PB | +1.490 |
| 5 | Karolina Gąsecka (B2) | POL | 41.65 | | +1.850 |
| 6 | Raluca Doina Ştef (Senior) | ROU | 41.71 | SB | +1.910 |
| 7 | Marta.02 Dobrowolska (C2) | POL | 42.13 | SB | +2.330 |
| 8 | Eline van Voorden (B1) | NED | 42.22 | | +2.420 |
| 9 | Inge de Heer (B1) | NED | 42.24 | SB | +2.440 |
| 10 | Ramona Härdi (Neo) | SUI | 42.25 | | +2.450 |
| 11 | Maja Płończyk (B1) | POL | 42.28 | | +2.480 |
| 12 | Sophie Warmuth (C1) | GER | 42.69 | SB | +2.890 |
| Katja Lutz (Senior) | SUI | 42.69 | | +2.890 |
| 14 | Vera Lou Gemser (C2) | NED | 42.73 | SB | +2.930 |
| 15 | Daphne de Ruiter (B1) | NED | 42.82 | | +3.020 |
| 16 | Naomi van der Werf (A1) | NED | 43.21 | SB | +3.410 |
| 17 | Klaudia Lorenc (B1) | POL | 43.72 | | +3.920 |
| 18 | Romée Ebbinge (A1) | NED | 43.74 | | +3.940 |
| 19 | Kiki Tellier (B1) | NED | 43.76 | | +3.960 |
| 20 | Anais Pedroni (B1) | ITA | 43.86 | | +4.060 |
| 21 | Mariya Irlina (C1) | GER | 44.43 | | +4.630 |
| 22 | Ludovica Boaretto (C1) | ITA | 44.49 | | +4.690 |
| 23 | Giada Nenzi (A2) | ITA | 44.51 | | +4.710 |
| 24 | Aurelia Mulsow (D2) | GER | 44.68 | SB | +4.880 |
| 25 | Julia Bachl (C1) | GER | 44.91 | SB | +5.110 |
| 26 | Josephine Mönnich (C2) | GER | 45.09 | | +5.290 |
| 27 | Urszula Mrugała (C2) | POL | 45.14 | | +5.340 |
| 28 | Caroline Möller (C1) | GER | 45.22 | SB | +5.420 |
| Julia Uziak (C1) | POL | 45.22 | | +5.420 |
| 30 | Diana Opengeym (D2) | GER | 45.26 | SB | +5.460 |
| 31 | Vanessa Nana Niznik (B1) | GER | 45.33 | | +5.530 |
| 32 | Lina Guzowski (B1) | GER | 45.70 | PB | +5.900 |
| 33 | Maria Dobosz (D2) | POL | 45.81 | | +6.010 |
| 34 | Melody Igrishta (C1) | GER | 45.91 | SB | +6.110 |
| 35 | Maira Jasch (D1) | GER | 46.01 | SB | +6.210 |
| 36 | Leonie Mayrhofer (D2) | GER | 46.13 | SB | +6.330 |
| 37 | Karolina Jagniszczak (B1) | POL | 46.25 | | +6.450 |
| 38 | Lina Rössler (C2) | GER | 46.42 | | +6.620 |
| 39 | Nyncke Dijkstra (C1) | NED | 46.48 | SB | +6.680 |
| 40 | Anna Bretschneider (D2) | GER | 46.77 | SB | +6.970 |
| 41 | Marlene Zink (D2) | GER | 46.86 | SB | +7.060 |
| Barbara Smolińska (C2) | POL | 46.86 | | +7.060 |
| 43 | Julia Marcjoniak (A1) | POL | 46.92 | | +7.120 |
| 44 | Maria Spantig (C2) | GER | 46.97 | | +7.170 |
| 45 | Jana Lunze (D2) | GER | 47.05 | | +7.250 |
| 46 | Donna Noor Gemser (E2) | NED | 47.09 | SB | +7.290 |
| 47 | Aurora Resmini (D2) | ITA | 47.18 | | +7.380 |
| 48 | Amelie Bretschneider (D2) | GER | 47.30 | | +7.500 |
| 49 | Maja Wagner (C2) | GER | 47.48 | | +7.680 |
| 50 | Lilly Passek (D2) | GER | 47.51 | | +7.710 |
| 51 | Clea Zimmermann (C2) | GER | 47.96 | PB | +8.160 |
| 52 | Cheyenne Schmidt (C1) | GER | 48.09 | | +8.290 |
| 53 | Matilde Rigoni (A1) | ITA | 48.12 | | +8.320 |
| 54 | Nina Mayrhofer (D2) | GER | 48.34 | SB | +8.540 |
| 55 | Antonia Luisa Rückmann (D2) | GER | 48.41 | | +8.610 |
| 56 | Amelia Stec (D1) | POL | 48.78 | | +8.980 |
| 57 | Isabel Kraus (D1) | GER | 49.40 | | +9.600 |
| 58 | Louisa Patz (D2) | GER | 49.41 | SB | +9.610 |
| 59 | Sophie Patz (E2) | GER | 49.95 | SB | +10.150 |
| 60 | Stella Marie Junge (E2) | GER | 50.88 | SB | +11.080 |
| 61 | Lena Arens (B1) | GER | 1:00.39 | | +20.590 |
| DNS | Marie Motschmann (C2) | GER | DNS | | - |
| DQ | Martyna Baran (B1) | POL | DQ | | - |