| 1 | Lea Sophie Scholz (Senior) | GER | 40.87 | SB | - |
| 2 | Laura Kivioja (Neo) | FIN | 41.21 | SB | +0.340 |
| 3 | Giorgia Fusetto (A2) | ITA | 41.24 | SB | +0.370 |
| 4 | Sofiia Khochyna (Neo) | UKR | 42.09 | SB | +1.220 |
| 5 | Sarah Rosner (B2) | AUT | 42.29 | PB | +1.420 |
| Marlen Ehseluns (Neo) | GER | 42.29 | PB | +1.420 |
| 7 | Nikol Nepokojova (Neo) | CZE | 42.44 | SB | +1.570 |
| 8 | Leia Marie Behlau (Senior) | GER | 42.88 | SB | +2.010 |
| 9 | Saskia Kütt (B2) | EST | 44.28 | SB | +3.410 |
| 10 | Patrīcija Elizabete Sniķere (Neo) | LAT | 44.53 | SB | +3.660 |
| 11 | Emilia Hopf (C2) | GER | 44.62 | SB | +3.750 |
| 12 | Adéla Krejčová (B2) | CZE | 44.86 | SB | +3.990 |
| 13 | Ema Schneiderova (A2) | CZE | 46.37 | SB | +5.500 |
| 14 | Sophia Kopecková (C1) | CZE | 46.43 | PB | +5.560 |
| 15 | Tereza Dolezalová (B1) | CZE | 46.49 | SB | +5.620 |
| 16 | Lilly Engelhardt (C2) | GER | 46.52 | PB | +5.650 |
| 17 | Lene Hilbig (D2) | GER | 47.83 | SB | +6.960 |
| 18 | Jázmin Csanádi (C1) | HUN | 47.90 | SB | +7.030 |
| 19 | Emma Engelhardt (D2) | GER | 48.44 | PB | +7.570 |
| 20 | Lucie Pecová (C1) | CZE | 49.68 | PB | +8.810 |
| 21 | Johanna Kogler (E2) | GER | 50.01 | PB | +9.140 |
| 22 | Martina Sedláčková (D2) | CZE | 50.19 | PB | +9.320 |
| 23 | Barbara Tóth (B1) | HUN | 51.47 | PB | +10.600 |
| 24 | Anna Zauner (D1) | GER | 51.74 | PB | +10.870 |
| 25 | Lieke Planting (D2) | GER | 52.32 | PB | +11.450 |
| 26 | Petra Zsiga (D2) | HUN | 52.52 | PB | +11.650 |
| 27 | Virág Kiss (D1) | HUN | 53.00 | PB | +12.130 |
| 28 | Elisa Spiegel (B2) | GER | 53.30 | SB | +12.430 |
| 29 | Lisandra Tarmet (B1) | EST | 53.68 | SB | +12.810 |
| 30 | Greta Schwabl (E1) | GER | 54.40 | SB | +13.530 |
| 31 | Emily Lai (D2) | CZE | 55.16 | SB | +14.290 |
| 32 | Helin Kuus (C1) | EST | 55.61 | PB | +14.740 |
| 33 | Maryna Osypenko (D1) | UKR | 55.88 | PB | +15.010 |
| 34 | Luna Heinle (C1) | GER | 56.70 | SB | +15.830 |
| 35 | Zuzana Dvorackova (C1) | CZE | 59.18 | PB | +18.310 |
| 36 | Ema Gaierová (D2) | CZE | 1:01.07 | SB | +20.200 |
| 37 | Ioana Stan (C2) | ROU | 1:01.92 | SB | +21.050 |
| 38 | Polina Dolbina (B1) | UKR | 1:04.18 | PB | +23.310 |
| DNF | Kertu Põldmaa (C1) | EST | DNF | | - |
| DNF | Luisa Koch (D1) | GER | DNF | | - |
| DQ | Aurora Aiello (B1) | ITA | DQ | | - |
| DNF | Sofiia Shelekhova (C2) | UKR | DNF | | - |
| WDR | Sofia Havryliuk (D1) | UKR | WDR | | - |