| 1 | Sofia Schilder (B2) | NED | 44.97 | | - |
| 2 | Sterre Kamphuis (A1) | NED | 44.98 | | +0.010 |
| 3 | Milou Butter (Neo) | NED | 45.71 | | +0.740 |
| 4 | Laura Linssen (A1) | NED | 45.78 | | +0.810 |
| 5 | Zhara Möllers (B1) | NED | 45.94 | | +0.970 |
| 6 | Milou van Leeuwen (Neo) | NED | 46.40 | | +1.430 |
| 7 | Danique van Rooden (B1) | NED | 46.82 | | +1.850 |
| 8 | Rianne Koot (Neo) | NED | 47.19 | | +2.220 |
| 9 | Lara Dingjan (A1) | NED | 47.35 | | +2.380 |
| 10 | Anouk Könst (B2) | NED | 48.36 | | +3.390 |
| 11 | Sanne Roos (A2) | NED | 48.39 | | +3.420 |
| 12 | Lotte de Bock (B2) | NED | 48.55 | | +3.580 |
| 13 | Aranka Keur (M40) | NED | 50.42 | | +5.450 |
| 14 | Marit.0608 van der Meer (C2) | NED | 50.55 | | +5.580 |
| 15 | Sanne Ganzinga (B2) | NED | 51.57 | | +6.600 |
| 16 | Tessa Ruissen (B2) | NED | 51.83 | PB | +6.860 |
| 17 | Juul Jansen (C2) | NED | 52.61 | | +7.640 |
| 18 | Emma Hartveld (A1) | NED | 52.65 | | +7.680 |
| 19 | Mette van Leeuwen (B1) | NED | 53.07 | | +8.100 |
| 20 | Annette Laarhoven (B1) | NED | 53.11 | | +8.140 |
| 21 | Valerie Nijman (B2) | NED | 53.74 | | +8.770 |
| 22 | Melanie van der Zalm (Neo) | NED | 54.15 | | +9.180 |
| 23 | Wieke Eikelenboom (B1) | NED | 54.52 | | +9.550 |
| 24 | Pien Lodder (A2) | NED | 54.84 | | +9.870 |
| 25 | Charline Coates-Kwadrin (B1) | GBR | 55.11 | | +10.140 |
| 26 | Eveline Krom (B2) | NED | 55.50 | | +10.530 |
| 27 | Tjitske Kikstra (Neo) | NED | 55.81 | | +10.840 |
| 28 | Lynn van der Slot (D2) | NED | 56.46 | | +11.490 |
| 29 | Kelly Tromp (Senior) | NED | 57.30 | | +12.330 |
| 30 | Judith van Hesselingen (M50) | NED | 58.31 | | +13.340 |
| 31 | Ramona van Leeuwen-van Noort (M50) | NED | 1:03.28 | | +18.310 |