| 1 | Milou Butter (Neo) | NED | 45.48 | SB | - |
| 2 | Zhara Möllers (A1) | NED | 46.13 | | +0.650 |
| 3 | Maureen Hagen (A2) | NED | 46.29 | | +0.810 |
| 4 | Renske van der Veer (Neo) | NED | 47.47 | | +1.990 |
| 5 | Bente Adema (B1) | NED | 47.84 | SB | +2.360 |
| 6 | Kersty Heeremans (Neo) | NED | 48.15 | | +2.670 |
| 7 | Aranka Keur (M40) | NED | 48.32 | | +2.840 |
| 8 | Tessa Dijksman (Senior) | NED | 48.36 | | +2.880 |
| 9 | Britt van Wijk (B2) | NED | 48.72 | | +3.240 |
| 10 | Lianne van Assema (B1) | NED | 49.45 | | +3.970 |
| 11 | Eline Schmutzer (B1) | NED | 49.46 | SB | +3.980 |
| 12 | Charline Coates-Kwadrin (A1) | GBR | 49.77 | | +4.290 |
| 13 | Ciska de Ree (M35) | NED | 49.91 | | +4.430 |
| 14 | Wende Heeremans (B2) | NED | 50.79 | | +5.310 |
| 15 | Renske Bruinsma (Senior) | NED | 50.82 | SB | +5.340 |
| 16 | Caroline Broekhuizen (A2) | NED | 51.35 | SB | +5.870 |
| 17 | Anne Lubbers (B2) | NED | 51.96 | | +6.480 |
| 18 | Annette Laarhoven (A1) | NED | 52.33 | | +6.850 |
| 19 | Franca Baars (B1) | NED | 53.12 | | +7.640 |
| 20 | Valerie Nijman (A2) | NED | 53.41 | | +7.930 |
| 21 | Mette van Leeuwen (A1) | NED | 53.63 | | +8.150 |
| 22 | Eveline Krom (A2) | NED | 55.06 | | +9.580 |
| 23 | Fabienne de Bie (A1) | NED | 55.24 | SB | +9.760 |
| 24 | Wieteke Huiberts (Neo) | NED | 55.83 | | +10.350 |
| 25 | Judith van Hesselingen (M55) | NED | 1:00.03 | | +14.550 |
| 26 | Mirthe Visser (B2) | NED | 1:00.65 | | +15.170 |
| 27 | Meggy van Dijk-Brolsma (M50) | NED | 1:02.92 | SB | +17.440 |