| 1 | Yuuma Murakami (Senior) | JPN | 34.44 | SB | - |
| 2 | Tatsuya Shinhama (Senior) | JPN | 34.51 | | +0.070 |
| 3 | Yamato Matsui (Neo) | JPN | 35.04 | | +0.600 |
| 4 | Ryota Kojima (Neo) | JPN | 35.17 | | +0.730 |
| 5 | Daichi Yamanaka (Senior) | JPN | 35.21 | | +0.770 |
| 6 | Tsubasa Hasegawa (Senior) | JPN | 35.40 | | +0.960 |
| 7 | Takuma Kawame (Senior) | JPN | 35.50 | | +1.060 |
| 8 | Keigo Abe (Senior) | JPN | 35.65 | | +1.210 |
| 9 | Yuuma Uehara (Senior) | JPN | 35.66 | | +1.220 |
| 10 | Ryouhei Haga (Senior) | JPN | 35.66 | | +1.222 |
| 11 | Masaya Yamada (Senior) | JPN | 35.83 | | +1.390 |
| 12 | Kazuya Anbo (Neo) | JPN | 35.90 | | +1.460 |
| 13 | Itsushi Tsujimoto (Senior) | JPN | 35.99 | | +1.550 |
| 14 | Junya Miwa (Senior) | JPN | 36.02 | | +1.580 |
| 15 | Shunsuke Nakamura (Senior) | JPN | 36.03 | | +1.590 |
| 16 | Shane Williamson (Senior) | JPN | 36.16 | SB | +1.720 |
| 17 | Kouki Kubo (Neo) | JPN | 36.19 | | +1.750 |
| 18 | Naoki Takezawa (A2) | JPN | 36.43 | | +1.990 |
| 19 | Shuuma Satake (Neo) | JPN | 36.66 | | +2.220 |
| 20 | Mahiro Hara (Neo) | JPN | 36.75 | | +2.310 |
| 21 | Shinnosuke Honma (Neo) | JPN | 36.90 | | +2.460 |
| 22 | Taisei Tawarayama (Neo) | JPN | 36.92 | SB | +2.480 |
| 23 | Tomoki Samekawa (B2) | JPN | 36.93 | SB | +2.490 |
| 24 | Reima Ugajin (Neo) | JPN | 36.99 | | +2.550 |
| 25 | Masahito Oobayashi (Senior) | JPN | 37.02 | | +2.580 |
| 26 | Tomonari Mitsuoka (Senior) | JPN | 37.07 | PB | +2.630 |
| 27 | Taki Shimizu (A2) | JPN | 37.16 | SB | +2.720 |
| 28 | Koudai Nagasaki (A1) | JPN | 37.22 | | +2.780 |
| 29 | Kousuke Ooyanagi (B2) | JPN | 37.37 | PB | +2.938 |
| 30 | Jin Akasaka (Neo) | JPN | 37.37 | | +2.939 |
| 31 | Kyouhei Matsumoto (Neo) | JPN | 37.49 | | +3.050 |
| 32 | Atsuro Sugiyama (Neo) | JPN | 37.64 | SB | +3.200 |
| 33 | Rikuya Iidzuka (Neo) | JPN | 37.85 | SB | +3.410 |
| 34 | Tatsuya Matsumoto (Neo) | JPN | 37.89 | | +3.450 |
| 35 | Masaya Suzuki (Neo) | JPN | 38.02 | | +3.580 |
| 36 | Souichiro Kumagai (C2) | JPN | 38.46 | | +4.020 |
| 37 | Jin Urakawa (C2) | JPN | 38.51 | SB | +4.070 |
| 38 | Takumi Murashita (C1) | JPN | 38.52 | SB | +4.080 |
| 39 | Kouki Oyama (A1) | JPN | 39.03 | | +4.590 |
| 40 | Shuuji Sugawara (M35) | JPN | 39.23 | | +4.790 |
| 41 | Yuuto.07 Sato (C1) | JPN | 39.27 | SB | +4.830 |
| 42 | Souta Yoshida (C2) | JPN | 39.28 | SB | +4.840 |
| 43 | Atsushi.06 Sato (C2) | JPN | 39.54 | SB | +5.100 |
| 44 | Daiki Sasaki (B1) | JPN | 39.87 | PB | +5.430 |
| 45 | Raimon Kato (C1) | JPN | 39.91 | | +5.470 |
| 46 | Yuu Hagiwara (A2) | JPN | 39.99 | | +5.550 |
| 47 | Haruto Abe (C2) | JPN | 40.21 | | +5.770 |
| 48 | Shinji Shimodate (Neo) | JPN | 40.48 | | +6.040 |
| 49 | Hisato Anda (C2) | JPN | 40.56 | | +6.120 |
| 50 | Kazuki Fujita (C2) | JPN | 40.62 | SB | +6.180 |
| 51 | Sho Kamada (B1) | JPN | 41.47 | SB | +7.030 |
| 52 | Taichi Hasegawa (C2) | JPN | 41.91 | SB | +7.470 |
| 53 | Hiroki Kashiwazaki (B1) | JPN | 41.93 | | +7.490 |
| 54 | Akira Kanehama (M30) | JPN | 42.08 | SB | +7.640 |
| 55 | Masato Taniguchi (C2) | JPN | 42.28 | | +7.840 |
| 56 | Atsushi Ogasawara (A2) | JPN | 42.59 | | +8.150 |
| 57 | Isshin Kohata (C1) | JPN | 43.00 | | +8.560 |
| 58 | Masataka Yamada (Neo) | JPN | 43.18 | PB | +8.740 |
| 59 | Masaya Endo (B1) | JPN | 44.59 | PB | +10.150 |
| 60 | Masaki Yamamoto (M45) | JPN | 44.84 | SB | +10.400 |
| 61 | Hayato Nakayama (D1) | JPN | 45.80 | SB | +11.360 |
| 62 | Kouyo Nagasaki (E2) | JPN | 46.00 | SB | +11.560 |
| 63 | Eiki Kawashima (M65) | JPN | 46.58 | SB | +12.140 |
| 64 | Hiroto Uetsu (D2) | JPN | 46.63 | SB | +12.190 |
| 65 | Yuu Takehana (D2) | JPN | 46.79 | SB | +12.350 |
| 66 | Kate Atomura (D1) | JPN | 46.81 | SB | +12.370 |
| 67 | Sayre Funaba (D2) | JPN | 47.25 | | +12.810 |
| 68 | Daito Anda (D1) | JPN | 47.27 | SB | +12.830 |
| 69 | Daiki Yamashita (C2) | JPN | 47.74 | SB | +13.300 |
| 70 | Koutaro Yonekawa (E2) | JPN | 48.15 | SB | +13.710 |
| 71 | Tatsuya Tanaka (D1) | JPN | 48.68 | SB | +14.240 |
| 72 | Reo Wada (D2) | JPN | 49.19 | SB | +14.750 |
| 73 | Daito Sato (C2) | JPN | 49.27 | SB | +14.830 |
| 74 | Shouma Shiroiwa (D2) | JPN | 49.58 | SB | +15.140 |
| 75 | Mikio Nagai (M50) | JPN | 49.95 | | +15.510 |
| 76 | Souta Matsuda (D2) | JPN | 50.04 | SB | +15.600 |
| 77 | Yuuri Sato (D1) | JPN | 58.16 | PB | +23.720 |
| 78 | Yousuke.08 Sasaki (D2) | JPN | 58.30 | SB | +23.860 |
| 79 | Kazuto Sato (D1) | JPN | 58.86 | SB | +24.420 |
| 80 | Kairi Sato (D1) | JPN | 58.95 | PB | +24.510 |
| 81 | Kenshin Kato (E1) | JPN | 1:03.58 | SB | +29.140 |
| 82 | Seiya Tanaka (D2) | JPN | 1:06.27 | SB | +31.830 |
| 83 | Mikoto Tanji (D1) | JPN | 1:06.95 | SB | +32.510 |
| WDR | Eikichi Sakamoto (Senior) | JPN | WDR | | - |
| DQ | Kouki Yokosawa (Neo) | JPN | DQ | | - |
| WDR | Tsukasa Nagane (M45) | JPN | WDR | | - |