| 1 | Blair Cruikshank (Senior) | USA | 40.49 | | - |
| 2 | Victoria Goplen (Neo) | CAN | 40.63 | PB | +0.140 |
| 3 | Piper Yde (Neo) | USA | 41.02 | SB | +0.530 |
| 4 | Sydney-Yu Terpening (Senior) | USA | 43.10 | | +2.610 |
| 5 | Nastasja Radulovic (A2) | USA | 44.13 | SB | +3.640 |
| 6 | Evie Forrer (B1) | USA | 44.26 | | +3.770 |
| 7 | Mirae Mastrolonardo (Senior) | USA | 44.62 | SB | +4.130 |
| 8 | Elliana Fickel (C2) | USA | 47.28 | | +6.790 |
| 9 | Natalie Rodrigue-Hasselback (M50) | USA | 47.66 | SB | +7.170 |
| 10 | Audrey Steffes (Neo) | USA | 47.79 | SB | +7.300 |
| 11 | Leah Pickett-Doty (n/a) | USA | 48.20 | PB | +7.710 |
| 12 | Barbara Johnson (M70) | USA | 49.69 | SB | +9.200 |
| 13 | Mary Murphy (n/a) | USA | 50.68 | PB | +10.190 |
| 14 | Jessica Chu (M30) | CAN | 51.16 | PB | +10.670 |
| 15 | Genevive Lemieux (M50) | CAN | 52.01 | SB | +11.520 |
| 16 | Katherine Denis (n/a) | CAN | 57.40 | PB | +16.910 |
| 17 | Hilles Hamersley (M60) | CAN | 1:00.36 | SB | +19.870 |
| 18 | Charlotte Ribecky (n/a) | USA | 1:00.89 | PB | +20.400 |