| 1 | Konami Soga (Senior) | JPN | 39.56 | SB | - |
| 2 | Leonie Bats (Neo) | NED | 40.68 | SB | +1.120 |
| 3 | Amy van der Meer (Neo) | NED | 40.95 | SB | +1.390 |
| 4 | Yasmine Bouaziz (Neo) | NED | 41.00 | SB | +1.440 |
| 5 | Sterre van Schaik (Neo) | NED | 41.05 | PB | +1.490 |
| 6 | Isa Leroy (A2) | NED | 41.18 | SB | +1.620 |
| 7 | Yael Prenger (Neo) | NED | 41.21 | SB | +1.650 |
| 8 | Lieke Hoogendoorn (A2) | NED | 41.34 | SB | +1.780 |
| 9 | Vera van Ditshuizen (Neo) | NED | 41.40 | PB | +1.840 |
| Rosalie van Vliet (A2) | NED | 41.40 | PB | +1.840 |
| 11 | Quinty Hersi (B2) | NED | 41.79 | SB | +2.230 |
| 12 | Sofie Bouw (Neo) | NED | 41.84 | SB | +2.280 |
| 13 | Maaike Koelewijn (A1) | NED | 41.85 | SB | +2.290 |
| 14 | Susanne Prins (Neo) | NED | 41.87 | SB | +2.310 |
| 15 | Marieke de Vries (Neo) | NED | 41.91 | SB | +2.350 |
| 16 | Amée Hollanders (Neo) | NED | 41.92 | SB | +2.360 |
| 17 | Lisan van der Linde (Neo) | NED | 42.04 | SB | +2.480 |
| 18 | Claretta Caravita (Senior) | NED | 42.40 | SB | +2.840 |
| 19 | Jasmijn ter Haar (B1) | NED | 42.47 | SB | +2.910 |
| 20 | Sanne Oosterwijk (Neo) | NED | 42.87 | SB | +3.310 |
| 21 | Emma Noz (Neo) | NED | 42.88 | SB | +3.320 |
| 22 | Lidia Tempert (Senior) | NED | 43.21 | SB | +3.650 |
| 23 | Anna Marit Sybrandi (Neo) | NED | 43.23 | SB | +3.670 |
| 24 | Gwen Konter (Neo) | NED | 43.74 | SB | +4.180 |
| 25 | Nynke Ytje Boonstra (Neo) | NED | 45.94 | SB | +6.380 |
| 26 | Lieve Bunt (A2) | NED | 47.44 | SB | +7.880 |