| 1 | Marije van der Spek (Neo) Marije van der Spek (Neo) | NED | 41.53 | SB | - |
| 2 | Tessa Oudman (Senior) Tessa Oudman (Senior) | NED | 41.63 | SB | +0.100 |
| 3 | Mara Vergeer (B2) Mara Vergeer (B2) | NED | 41.67 | PB | +0.140 |
| 4 | Lataesha Narain (Neo) Lataesha Narain (Neo) | NED | 41.70 | SB | +0.170 |
| 5 | Charley Boekema (D2) Charley Boekema (D2) | NED | 41.71 | SB | +0.180 |
| 6 | Jasmijn ter Haar (B1) Jasmijn ter Haar (B1) | NED | 41.78 | SB | +0.250 |
| 7 | Laura Linssen (Neo) Laura Linssen (Neo) | NED | 42.86 | SB | +1.330 |
| Elise Favre (B2) Elise Favre (B2) | NED | 42.86 | SB | +1.330 |
| 9 | Jasmin van der Terp (A2) Jasmin van der Terp (A2) | NED | 42.93 | SB | +1.400 |
| 10 | Nienke Meesters (C2) Nienke Meesters (C2) | NED | 43.31 | PB | +1.780 |
| 11 | Britt Post (B1) Britt Post (B1) | NED | 43.38 | PB | +1.850 |
| 12 | Emilie Fransen (B2) Emilie Fransen (B2) | NED | 43.73 | SB | +2.200 |
| 13 | Danique Happel (Neo) Danique Happel (Neo) | NED | 43.76 | SB | +2.230 |
| 14 | Danique van Rooden (A1) Danique van Rooden (A1) | NED | 43.81 | SB | +2.280 |
| 15 | Jitte Schuitemaker (Neo) Jitte Schuitemaker (Neo) | NED | 43.92 | PB | +2.390 |
| Sigrid van Es (D2) Sigrid van Es (D2) | NED | 43.92 | PB | +2.390 |
| 17 | Cleo Elenbaas (A2) Cleo Elenbaas (A2) | NED | 44.03 | SB | +2.500 |
| 18 | Fabienne Maarse (B2) Fabienne Maarse (B2) | NED | 44.04 | SB | +2.510 |
| 19 | Liv Kogelman (C2) Liv Kogelman (C2) | NED | 44.10 | SB | +2.570 |
| 20 | Annick van der Woude (Neo) Annick van der Woude (Neo) | NED | 44.25 | PB | +2.720 |
| 21 | Lena de Groot (B1) Lena de Groot (B1) | NED | 44.31 | PB | +2.780 |
| 22 | Lotte Welboren (B1) Lotte Welboren (B1) | NED | 44.64 | SB | +3.110 |
| 23 | Janne Boos (A1) Janne Boos (A1) | NED | 44.66 | SB | +3.130 |
| 24 | Pauline Tas (B1) Pauline Tas (B1) | NED | 45.08 | SB | +3.550 |
| 25 | Mariel Mur (B1) Mariel Mur (B1) | NED | 45.24 | PB | +3.710 |
| 26 | Fiore Koutstaal (C2) Fiore Koutstaal (C2) | NED | 45.43 | SB | +3.900 |
| 27 | Iris Verbeek (A1) Iris Verbeek (A1) | NED | 45.46 | SB | +3.930 |
| 28 | Imre Blomberg (D2) Imre Blomberg (D2) | NED | 45.54 | PB | +4.010 |
| 29 | Quinty Bloedjes (B2) Quinty Bloedjes (B2) | NED | 45.64 | PB | +4.110 |
| 30 | Evi Blomberg (C2) Evi Blomberg (C2) | NED | 45.95 | SB | +4.420 |
| 31 | Noor van der Peet (B1) Noor van der Peet (B1) | NED | 45.96 | SB | +4.430 |
| Asia Berga (C2) Asia Berga (C2) | NED | 45.96 | SB | +4.430 |
| 33 | Bregje Nagelmaeker (A2) Bregje Nagelmaeker (A2) | NED | 45.97 | SB | +4.440 |
| 34 | Anne Breider (C2) Anne Breider (C2) | NED | 46.17 | SB | +4.640 |
| 35 | Ava Zachrisson (C1) Ava Zachrisson (C1) | NED | 46.57 | PB | +5.040 |
| 36 | Bo Meijer (Neo) Bo Meijer (Neo) | NED | 46.68 | SB | +5.150 |
| 37 | Veerle Zunnebeld (C1) Veerle Zunnebeld (C1) | NED | 46.88 | SB | +5.350 |
| 38 | Charissa de Mes (B1) Charissa de Mes (B1) | NED | 46.93 | PB | +5.400 |
| 39 | Noor Wiering (B1) Noor Wiering (B1) | NED | 47.20 | PB | +5.670 |
| 40 | Anne-Fleur van der Lied (A1) Anne-Fleur van der Lied (A1) | NED | 47.25 | SB | +5.720 |
| 41 | Ilse Hofstee (Neo) Ilse Hofstee (Neo) | NED | 47.94 | PB | +6.410 |
| 42 | Sjuul Te Braake (C1) Sjuul Te Braake (C1) | NED | 48.40 | PB | +6.870 |
| 43 | Naomi Segers (D2) Naomi Segers (D2) | NED | 48.45 | PB | +6.920 |
| 44 | Femke Lindeboom (C1) Femke Lindeboom (C1) | NED | 48.48 | SB | +6.950 |
| 45 | Denise Bakker (Neo) Denise Bakker (Neo) | NED | 48.92 | PB | +7.390 |
| 46 | Jonne Holthuis (C1) Jonne Holthuis (C1) | NED | 48.98 | SB | +7.450 |
| 47 | Florine Zunnebeld (D2) Florine Zunnebeld (D2) | NED | 51.42 | PB | +9.890 |
| WDR | Henny de Vries (Neo) Henny de Vries (Neo) | NED | WDR | | - |
| DNF | Marit Veenhuis (C1) Marit Veenhuis (C1) | NED | DNF | | - |
| WDR | Anniek Heethaar (B2) Anniek Heethaar (B2) | NED | WDR | | - |
| DQ | Marleen Knol (B2) Marleen Knol (B2) | NED | DQ | | - |