| 1 | Susan Bol (Neo) | NED | 46.46 | | - |
| 2 | Tamara Zwaan (Neo) | NED | 46.76 | | +0.300 |
| 3 | Geertruida van Wonderen (Senior) | BEL | 46.86 | SB | +0.400 |
| 4 | Berdy Kars (Senior) | NED | 47.27 | | +0.810 |
| 5 | Nienke Stumpel (Neo) | NED | 47.98 | | +1.520 |
| 6 | Jannemiek Evelo (A2) | NED | 48.61 | SB | +2.150 |
| 7 | Esther Zwaan (Neo) | NED | 49.36 | SB | +2.900 |
| 8 | Meike Hoogeboom (Neo) | NED | 50.29 | | +3.830 |
| 9 | Annelou Mayenburg (Neo) | NED | 51.78 | SB | +5.320 |
| 10 | Merel Meeuwes (Neo) | NED | 52.29 | SB | +5.830 |
| 11 | Lineke Oosterwechel (Senior) | NED | 53.15 | SB | +6.690 |
| 12 | Brechtje-Rinkje de Boer (A2) | NED | 53.86 | | +7.400 |
| 13 | Marieke Scheurs (A1) | NED | 54.01 | SB | +7.550 |
| 14 | Jeske Hoogeboom (C2) | NED | 54.28 | | +7.820 |
| 15 | Janneke Oostenrijk (Neo) | NED | 54.64 | SB | +8.180 |
| 16 | Chloe Hezemans (Senior) | NED | 54.81 | | +8.350 |
| 17 | Merel Birnie (n/a) | NED | 55.02 | SB | +8.560 |
| 18 | Veerle van Balen (Neo) | NED | 55.09 | SB | +8.630 |
| 19 | Marielle van Aalst (Neo) | NED | 56.89 | | +10.430 |
| 20 | Maartje Schuurman (A2) | NED | 57.56 | | +11.100 |
| 21 | Valerie Franke (n/a) | NED | 59.17 | SB | +12.710 |
| 22 | Kim Walravens (Senior) | NED | 1:05.68 | SB | +19.220 |
| 23 | Anneke van der Reijden (n/a) | NED | 1:08.24 | SB | +21.780 |
| 24 | Irene Bakker (n/a) | NED | 1:08.68 | SB | +22.220 |
| 25 | Wietske Waterlander (n/a) | NED | 1:10.03 | SB | +23.570 |
| 26 | Esther van Duin (n/a) | NED | 1:10.32 | PB | +23.860 |
| 27 | Anne Jentink (n/a) | NED | 1:11.61 | PB | +25.150 |
| 28 | Iris Hoogendoorn (n/a) | NED | 1:13.04 | SB | +26.580 |
| 29 | Marieke Bakker (M45) | NED | 1:13.16 | PB | +26.700 |
| 30 | Dionne Noordhof (n/a) | NED | 1:14.37 | SB | +27.910 |
| DNS | Liselotte Verdoorn (Neo) | NED | DNS | | - |