| 1 | Valerie Vinken (A1) | NED | 46.99 | | - |
| 2 | Sophie Moerman (A2) | NED | 48.62 | | +1.630 |
| 3 | Emilie Verberne (C2) | NED | 49.46 | | +2.470 |
| 4 | Yara Kok (B1) | NED | 50.23 | SB | +3.240 |
| 5 | Anne-Linde Paans (Senior) | NED | 50.71 | SB | +3.720 |
| 6 | Renee Schipper (Senior) | NED | 51.20 | PB | +4.210 |
| 7 | Milou Lammerts (Neo) | NED | 51.40 | | +4.410 |
| 8 | Elise Groenendaal (Senior) | NED | 52.14 | SB | +5.150 |
| 9 | Emma van der Zijl (n/a) | NED | 52.38 | PB | +5.390 |
| 10 | Yara van Wieringen (Senior) | NED | 52.88 | SB | +5.890 |
| 11 | Fien van Hall (D2) | NED | 54.11 | | +7.120 |
| 12 | Sophie Emans (n/a) | NED | 54.16 | PB | +7.170 |
| 13 | Bo Koolen (Neo) | NED | 54.29 | SB | +7.300 |
| 14 | Sterre Kuipers (Senior) | NED | 55.35 | SB | +8.360 |
| 15 | Talitha van Zuilen (Senior) | NED | 55.77 | SB | +8.780 |
| 16 | Vivian Gerretsen (n/a) | NED | 55.86 | PB | +8.870 |
| 17 | Tessa Schoon (Senior) | NED | 56.01 | SB | +9.020 |
| 18 | Anneloes Teunisse (Neo) | NED | 56.56 | SB | +9.570 |
| 19 | Jitte Schuitemaker (A2) | NED | 56.98 | | +9.990 |
| 20 | Julia Colombijn (n/a) | NED | 57.49 | PB | +10.500 |
| 21 | Jade Foppes (n/a) | NED | 57.71 | PB | +10.720 |
| 22 | Mimi van Hall (B2) | NED | 57.74 | | +10.750 |
| 23 | Elisabeth Jongsma (M55) | NED | 58.53 | SB | +11.540 |
| 24 | Lotte Smit (Senior) | NED | 58.69 | SB | +11.700 |
| 25 | Julia Oosenburg (Neo) | NED | 59.46 | SB | +12.470 |
| 26 | Isabella Flore (Neo) | NED | 59.50 | | +12.510 |
| 27 | Heleen Truijens (n/a) | NED | 59.79 | PB | +12.800 |
| 28 | Ezra Visser (B2) | NED | 1:00.08 | PB | +13.090 |
| 29 | Nine Walta (A2) | NED | 1:00.64 | SB | +13.650 |
| 30 | Rosa Smals (C2) | NED | 1:00.68 | | +13.690 |
| 31 | Ilja de Vries (M55) | NED | 1:01.02 | SB | +14.030 |
| 32 | Vera van de Meeberg (n/a) | NED | 1:02.63 | PB | +15.640 |
| 33 | Fieke Schilstra (n/a) | NED | 1:04.91 | SB | +17.920 |
| 34 | Roxy Bugter (Neo) | NED | 1:06.12 | SB | +19.130 |
| 35 | Daya Bakker (n/a) | NED | 1:07.31 | PB | +20.320 |
| 36 | Isa Spoelder (Senior) | NED | 1:13.09 | SB | +26.100 |
| 37 | Tine van Twillert (n/a) | NED | 1:13.67 | PB | +26.680 |
| 38 | Cira Klercq (B1) | NED | 1:19.00 | SB | +32.010 |
| 39 | Milou Ruurs (A2) | NED | 1:19.33 | SB | +32.340 |
| 40 | Emma Fransen (Neo) | NED | 1:20.79 | | +33.800 |
| DNS | Guusje Zautsen (Neo) | NED | DNS | | - |
| DNS | Loïs Rump (n/a) | NED | DNS | | - |
| DNS | Emma Malan-Revell (n/a) | NED | DNS | | - |