| 1 | Roos van den Bos (A1) | NED | 44.98 | | - |
| 2 | Joyce Stijger (A2) | NED | 45.16 | SB | +0.180 |
| 3 | Laura Milatz (B1) | NED | 47.00 | | +2.020 |
| 4 | Lieneke de Kroon (Neo) | NED | 47.14 | | +2.160 |
| 5 | Julia Eshuis (A2) | NED | 47.40 | PB | +2.420 |
| 6 | Eva Peters (A1) | NED | 47.58 | | +2.600 |
| 7 | Bernadette Jeremiasse (A1) | NED | 47.61 | | +2.630 |
| 8 | Suzanne Beets (B2) | NED | 47.84 | | +2.860 |
| 9 | Manon Floris (C1) | NED | 48.27 | | +3.290 |
| 10 | Sanne Smit (A1) | NED | 49.17 | | +4.190 |
| 11 | Louise van der Hijden (Senior) | NED | 49.99 | | +5.010 |
| 12 | Xanthe Jeremiasse (C1) | NED | 50.14 | | +5.160 |
| 13 | Tyrza Mollee (C1) | NED | 50.70 | | +5.720 |
| 14 | Yvette Molenaar (B2) | NED | 50.75 | SB | +5.770 |
| 15 | Rosa Steffens (C1) | NED | 51.19 | | +6.210 |
| 16 | Eveline Sikking (B1) | NED | 51.20 | | +6.220 |
| 17 | Anouk van Westerhoven (C1) | NED | 51.77 | | +6.790 |
| 18 | Nina Beumken (B1) | NED | 51.92 | | +6.940 |
| 19 | Sascha Leurs (Neo) | NED | 51.99 | | +7.010 |
| 20 | Carmen van den Hoogen (C2) | NED | 52.84 | | +7.860 |
| 21 | Nicole van Opbergen (C1) | NED | 53.01 | | +8.030 |
| 22 | Roxanne Ippel (A1) | NED | 53.27 | | +8.290 |
| 23 | Charity Post (B1) | NED | 54.00 | SB | +9.020 |
| 24 | Famke Wilmink (E2) | NED | 54.99 | | +10.010 |
| 25 | Renate Grigoleit (C2) | NED | 57.64 | PB | +12.660 |
| 26 | Emma Kostelijk (E2) | NED | 57.93 | | +12.950 |
| 27 | Sofie van der Hijden (Neo) | NED | 59.67 | | +14.690 |
| 28 | Melloney Post (D2) | NED | 1:03.30 | | +18.320 |
| 29 | Melanie Walters (D1) | NED | 1:42.40 | | +57.420 |