| 1 | Kimi Goetz (Senior) | USA | 38.02 | | - |
| 2 | Brittany Bowe (Senior) | USA | 38.05 | | +0.030 |
| 3 | Erin Jackson (Senior) | USA | 38.28 | | +0.260 |
| 4 | Vanessa Herzog (Senior) | AUT | 38.60 | | +0.580 |
| 5 | Sofia Thorup-Prosvirnova (Senior) | DEN | 38.86 | NR | +0.840 |
| 6 | McKenzie Browne (Senior) | USA | 39.03 | | +1.010 |
| 7 | Greta Myers (Neo) | USA | 39.62 | | +1.600 |
| 8 | Mia Manganello (Senior) | USA | 40.39 | | +2.370 |
| 9 | Libby Williams (Neo) | USA | 41.34 | | +3.320 |
| 10 | Ilsa Melin Sandvig Shobe (Senior) | USA | 41.63 | | +3.610 |
| 11 | Shruti Kotwal (M30) | IND | 42.50 | SB | +4.480 |
| 12 | Karen Lizeth Siatama Valencia (A2) | COL | 43.09 | | +5.070 |
| 13 | Larissa Paes (Senior) | BRA | 43.80 | | +5.780 |
| 14 | Cynthia Jennings (Senior) | USA | 44.32 | | +6.300 |
| 15 | Amy Acosta (Neo) | ECU | 44.40 | NR | +6.380 |
| 16 | Dasha Epperson (M40) | USA | 51.73 | | +13.710 |