| 1 | Sayuri Yoshii (Senior) | JPN | 37.55 | PB | - |
| 2 | Jekaterina Malysjeva (Neo) | RUS | 38.29 | SB | +0.740 |
| 3 | Shihomi Shinya (Senior) | JPN | 38.38 | | +0.830 |
| 4 | Tomomi Okazaki (M35) | JPN | 38.39 | | +0.840 |
| 5 | Heather Richardson (Neo) | USA | 38.42 | SB | +0.870 |
| 6 | Jekaterina Lobysjeva (Senior) | RUS | 38.56 | SB | +1.010 |
| 7 | Sayuri Oosuga (Senior) | JPN | 38.60 | | +1.050 |
| 8 | Monique Angermüller (Senior) | GER | 38.65 | SB | +1.100 |
| 9 | Natasja Bruintjes (Neo) | NED | 38.68 | SB | +1.130 |
| 10 | Thijsje Oenema (Neo) | NED | 38.70 | | +1.150 |
| 11 | Heike Hartmann (Senior) | GER | 38.73 | SB | +1.180 |
| 12 | Margot Boer (Neo) | NED | 38.74 | | +1.190 |
| 13 | Annette Gerritsen (Neo) | NED | 38.78 | | +1.230 |
| 14 | Elli Ochowicz (Senior) | USA | 38.90 | | +1.350 |
| 15 | Jennifer Rodriguez (Senior) | USA | 38.93 | SB | +1.380 |
| 16 | Laurine van Riessen (Neo) | NED | 39.34 | | +1.790 |
| 17 | Paulina Wallin (Senior) | SWE | 39.40 | | +1.850 |
| 18 | Alla Sjabanova (Senior) | RUS | 39.47 | SB | +1.920 |
| 19 | Marianne Timmer (Senior) | NED | 39.68 | | +2.130 |
| 20 | Rebekah Bradford (Senior) | USA | 39.79 | | +2.240 |
| 21 | Lauren Cholewinski (Neo) | USA | 39.86 | | +2.310 |
| 22 | Daniela Anschütz-Thoms (Senior) | GER | 39.96 | SB | +2.410 |
| 23 | Marrit Leenstra (Neo) | NED | 40.13 | | +2.580 |
| 24 | Galina Likhatsjova (Senior) | RUS | 40.30 | SB | +2.750 |
| 25 | Lisette van der Geest (Senior) | NED | 40.73 | | +3.180 |
| 26 | Kelly Gunther (Neo) | USA | 40.74 | | +3.190 |
| 27 | Netanya Mintz (Senior) | USA | 41.11 | SB | +3.560 |
| 28 | Ashlee Barnett (Neo) | USA | 41.54 | | +3.990 |
| 29 | Rebecca Lang (Senior) | USA | 41.63 | | +4.080 |
| 30 | Stephanie Beckert (Neo) | GER | 42.41 | SB | +4.860 |
| 31 | Jerica Tandiman (C1) | USA | 42.97 | | +5.420 |
| 32 | Sara Nielson (A2) | USA | 45.44 | | +7.890 |