| 1 | Karolína Erbanová (A2) | CZE | 38.62 | | - |
| 2 | Janine Smit (Neo) | NED | 38.79 | SB | +0.170 |
| 3 | Kate Hanly (A1) | CAN | 39.58 | | +0.960 |
| 4 | Diane Valkenburg (Senior) | NED | 39.92 | SB | +1.300 |
| 5 | Martina Sáblíková (Senior) | CZE | 40.16 | SB | +1.540 |
| 6 | Paulien van Deutekom (Senior) | NED | 40.19 | SB | +1.570 |
| 7 | Jennessa Kemp (Neo) | CAN | 40.62 | | +2.000 |
| 8 | Heather McLean (A2) | CAN | 40.74 | | +2.120 |
| 9 | Alexandra Ianculescu (Neo) | CAN | 41.44 | | +2.820 |
| 10 | Sara Hopkins (M30) | CAN | 41.48 | | +2.860 |
| 11 | Keira Poelzer (A2) | CAN | 41.50 | | +2.880 |
| 12 | Elyce Henry (Senior) | CAN | 41.58 | | +2.960 |
| 13 | Kelly Prokopchuk (Neo) | CAN | 41.64 | | +3.020 |
| 14 | Allison Banwell (A2) | CAN | 41.69 | | +3.070 |
| 15 | Véronique Huot (Neo) | CAN | 41.79 | | +3.170 |
| 16 | Hillary Fast (A2) | CAN | 42.30 | | +3.680 |
| 17 | Gillian Richmond (Neo) | CAN | 42.34 | | +3.720 |
| 18 | Ashley Nelson (Neo) | CAN | 42.90 | | +4.280 |
| 19 | Lillian Iwaniszyn (A1) | POL | 43.18 | | +4.560 |
| 20 | Katie Babich (A1) | CAN | 43.37 | | +4.750 |
| 21 | Jamie MacDonald (B2) | CAN | 43.55 | | +4.930 |
| 22 | Sarah Pousette (Neo) | CAN | 43.62 | | +5.000 |
| 23 | Lauren McGuire (Neo) | CAN | 43.63 | | +5.010 |
| 24 | Rosie Tempest (Neo) | CAN | 43.90 | | +5.280 |
| 25 | Ana Pacanins (Neo) | CAN | 44.81 | SB | +6.190 |
| 26 | Kelsey Reilly (A2) | CAN | 44.95 | | +6.330 |
| 27 | Anastasia Vogl (Neo) | CAN | 45.23 | | +6.610 |
| 28 | Elizabeth Gordon (Neo) | CAN | 46.02 | | +7.400 |
| 29 | Stacey Muise (Neo) | CAN | 46.87 | | +8.250 |