| 1 | Brittany Bowe (Senior) | USA | 37.59 | SB | - |
| 2 | Kimi Goetz (Senior) | USA | 37.70 | | +0.110 |
| 3 | Erin Jackson (Senior) | USA | 38.79 | | +1.200 |
| 4 | Maria Victoria Rodriguez (Senior) | ARG | 38.95 | | +1.360 |
| 5 | Brianna Bocox (Neo) | USA | 39.05 | | +1.460 |
| 6 | Chrysta Rands (Neo) | USA | 39.54 | SB | +1.950 |
| 7 | Paige Schwartzburg (Senior) | USA | 39.55 | SB | +1.960 |
| 8 | Mia Kilburg-Manganello (Senior) | USA | 40.93 | SB | +3.340 |
| 9 | Sarah Warren (Senior) | USA | 41.64 | | +4.050 |
| 10 | McKenzie Browne (Senior) | USA | 41.80 | | +4.210 |
| 11 | Jenni Kukkonen (Senior) | FIN | 42.58 | | +4.990 |
| 12 | Dessie Weigel (Senior) | USA | 42.63 | | +5.040 |
| 13 | Valentina Espinosa (Neo) | ARG | 44.08 | | +6.490 |
| 14 | Larissa Paes (Senior) | BRA | 44.08 | | +6.490 |
| 15 | Petra Acker (Senior) | USA | 44.32 | SB | +6.730 |
| 16 | Cynthia Jennings (Senior) | USA | 45.20 | | +7.610 |
| 17 | Shruti Kotwal (Senior) | IND | 46.65 | | +9.060 |
| 18 | Ema Hinckley (A2) | POR | 47.07 | NRJ | +9.480 |
| 19 | Vanessa Enriquez (Senior) | COL | 48.87 | | +11.280 |
| 20 | Ruthann Reed (n/a) | USA | 1:09.16 | | +31.570 |