| 1 | Carolina Hiller (Senior) | CAN | 38.62 | SB | - |
| 2 | Jenna Larter (Neo) | CAN | 39.04 | SB | +0.420 |
| 3 | Brooklyn McDougall (Senior) | CAN | 39.65 | SB | +1.030 |
| 4 | Alexa Scott (Senior) | CAN | 39.92 | SB | +1.300 |
| 5 | Maddison Pearman (Senior) | CAN | 39.98 | SB | +1.360 |
| 6 | Hyunseo Chung (Neo) | KOR | 40.70 | SB | +2.080 |
| 7 | In-Ah Choi (B1) | KOR | 40.80 | PB | +2.180 |
| 8 | Abigail Mccluskey (Senior) | CAN | 40.87 | SB | +2.250 |
| 9 | Soo-Yeon Lee (Neo) | KOR | 40.89 | SB | +2.270 |
| 10 | Yoo-Jin Yoon (B2) | KOR | 40.98 | SB | +2.360 |
| 11 | Darby Beeson (A2) | CAN | 41.02 | PB | +2.400 |
| 12 | Zhiyuan Wan (C2) | CHN | 41.54 | PB | +2.920 |
| 13 | Emma Langstrom (Neo) | CAN | 41.68 | SB | +3.060 |
| 14 | Laura Hall (Neo) | CAN | 41.79 | SB | +3.170 |
| 15 | Maya Lueders (A2) | CAN | 41.85 | SB | +3.230 |
| 16 | Seo-Hyeon Park (B1) | KOR | 41.86 | PB | +3.240 |
| 17 | Anna Bourgeois (Senior) | CAN | 41.97 | SB | +3.350 |
| 18 | Rachel Mallard (Neo) | CAN | 42.45 | SB | +3.830 |
| 19 | Blanche Cardinal Tremblay (A2) | CAN | 42.47 | SB | +3.850 |
| 20 | Claire Mallard (Neo) | CAN | 42.67 | SB | +4.050 |
| 21 | Kiersten Hagen (Senior) | CAN | 42.76 | SB | +4.140 |
| 22 | Aoife Marshall (Neo) | CAN | 42.78 | SB | +4.160 |
| 23 | Silke Kabel (B2) | NED | 43.19 | SB | +4.570 |
| 24 | Seo-Yeon Yoon (A1) | KOR | 44.05 | PB | +5.430 |
| 25 | Mary Brophy (M50) | USA | 44.82 | SB | +6.200 |
| 26 | Lindsay Smart (A2) | CAN | 45.08 | SB | +6.460 |
| 27 | Hannah North (Neo) | CAN | 45.21 | SB | +6.590 |
| 28 | Sophia Major (B2) | CAN | 45.24 | SB | +6.620 |
| 29 | Isabel Erasmus (A2) | CAN | 46.52 | SB | +7.900 |
| 30 | Kate Folmer (B1) | CAN | 47.13 | SB | +8.510 |
| 31 | Maya Kemp (A2) | CAN | 47.17 | SB | +8.550 |
| 32 | Margaret Breedlove (B2) | CAN | 48.37 | SB | +9.750 |
| 33 | Jillian Reeves (A2) | CAN | 49.74 | SB | +11.120 |