| 1 | Nao Kodaira (Senior) | JPN | 38.11 | | - |
| 2 | Annette Gerritsen (Senior) | NED | 38.69 | SB | +0.580 |
| 3 | Marrit Leenstra (Senior) | NED | 38.83 | | +0.720 |
| 4 | Mayon Kuipers (Senior) | NED | 38.93 | | +0.820 |
| 5 | Janine Smit (Senior) | NED | 39.03 | | +0.920 |
| 6 | Floor van den Brandt (Senior) | NED | 39.13 | | +1.020 |
| 7 | Rosa Pater (Senior) | NED | 39.14 | SB | +1.030 |
| 8 | Sanneke de Neeling (Neo) | NED | 39.24 | | +1.130 |
| 9 | Margot Boer (Senior) | NED | 39.27 | | +1.160 |
| 10 | Anice Das (Senior) | NED | 39.33 | | +1.220 |
| 11 | Bo van der Werff (Neo) | NED | 39.45 | | +1.340 |
| 12 | Roxanne van Hemert (Senior) | NED | 39.63 | | +1.520 |
| 13 | Bente van den Berge (Neo) | NED | 39.70 | | +1.590 |
| 14 | Linda de Vries (Senior) | NED | 39.82 | | +1.710 |
| 15 | Tessa Boogaard (Neo) | NED | 39.98 | SB | +1.870 |
| 16 | Jorien Voorhuis (Senior) | NED | 40.07 | SB | +1.960 |
| 17 | Sanne van der Schaar (Neo) | NED | 40.26 | | +2.150 |
| 18 | Lotte van Beek (Senior) | NED | 40.27 | | +2.160 |
| 19 | Maud van der Meer (Neo) | NED | 40.29 | | +2.180 |
| 20 | Aveline Hylkema (Neo) | NED | 40.29 | | +2.180 |
| 21 | Carlijn Achtereekte (Senior) | NED | 40.47 | SB | +2.360 |
| 22 | Diane Valkenburg (Senior) | NED | 40.68 | | +2.570 |
| 23 | Leeyen Harteveld (Neo) | NED | 40.80 | | +2.690 |
| 24 | Reina Anema (Neo) | NED | 40.87 | SB | +2.760 |
| 25 | Annemarie Boer (Neo) | NED | 40.88 | | +2.770 |
| 26 | Manouk van Tol (Senior) | NED | 41.11 | | +3.000 |
| 27 | Miranda Dekker (Senior) | NED | 41.21 | | +3.100 |
| 28 | Roza Blokker (Senior) | NED | 41.27 | SB | +3.160 |
| 29 | Julia Berentschot (Neo) | NED | 41.35 | | +3.240 |
| 30 | Demi van Benthem (A1) | NED | 41.54 | | +3.430 |
| 31 | Irene Schouten (Senior) | NED | 41.58 | | +3.470 |
| 32 | Natasja Roest (Neo) | NED | 41.64 | | +3.530 |
| 33 | Freya Reitsma (A1) | NED | 41.71 | | +3.600 |
| 34 | Inge Mostert (Neo) | NED | 41.95 | SB | +3.840 |
| 35 | Aafke Soet (A1) | NED | 42.06 | | +3.950 |
| 36 | Iza Stekelenburg (A2) | NED | 42.16 | SB | +4.050 |
| 37 | Rosanne Fischer (A2) | NED | 42.54 | | +4.430 |
| 38 | Linda Halling (A2) | NED | 42.60 | PB | +4.490 |
| DQ | Karolína Erbanová (Neo) | CZE | DQ | | - |