| 1 | Janine Garretsen (A1) | NED | 42.59 | | - |
| 2 | Evi de Ruiter (A1) | NED | 43.02 | | +0.430 |
| 3 | Maud Biezen (A1) | NED | 43.36 | | +0.770 |
| 4 | Naomi Wessels (B1) | NED | 44.25 | SB | +1.660 |
| 5 | Florine Hehewerth (Neo) | NED | 45.07 | SB | +2.480 |
| 6 | Elise Kortleve (A1) | NED | 46.07 | | +3.480 |
| 7 | Yara van Klaveren (B2) | NED | 46.86 | SB | +4.270 |
| 8 | Sarina van Dijk (Senior) | NED | 47.18 | | +4.590 |
| 9 | Isa Boer (B2) | NED | 48.28 | | +5.690 |
| 10 | Nicky de Looze (Senior) | NED | 48.62 | | +6.030 |
| 11 | Meike Wagemakers (Neo) | NED | 49.01 | | +6.420 |
| 12 | Anja Bollaart (M55) | NED | 49.64 | | +7.050 |
| 13 | Evelien Verschuuren (Neo) | NED | 49.83 | SB | +7.240 |
| 14 | Ilse van Ipenburg (M30) | NED | 50.46 | | +7.870 |
| 15 | Ciska Stark (M60) | NED | 50.99 | | +8.400 |
| 16 | Nynke van Rijs (A1) | NED | 51.64 | | +9.050 |
| 17 | Marianne de Neeling (M65) | NED | 53.15 | | +10.560 |
| 18 | Manoa Damminga (B1) | NED | 53.53 | SB | +10.940 |
| 19 | Claudia Vermeirsch (Senior) | BEL | 54.55 | SB | +11.960 |
| 20 | Ethel Pennings-Graat (M50) | NED | 1:07.89 | SB | +25.300 |