| 1 | Chrysta Rands-Evans (Senior) | USA | 38.48 | SB | - |
| 2 | McKenzie Browne (Senior) | USA | 39.10 | | +0.620 |
| 3 | Sarah Warren (Senior) | USA | 39.12 | SB | +0.640 |
| 4 | Blair Cruikshank (Senior) | USA | 39.71 | SB | +1.230 |
| 5 | Maria Victoria Rodriguez (Senior) | ARG | 39.73 | | +1.250 |
| 6 | Piper Yde (Neo) | USA | 40.57 | PB | +2.090 |
| Emma de Bock (B1) | USA | 40.57 | PB | +2.090 |
| 8 | Anna Quinn (Senior) | USA | 40.84 | | +2.360 |
| 9 | Libby Williams (Neo) | USA | 40.90 | SB | +2.420 |
| 10 | Ilsa Melin Sandvig Shobe (Senior) | USA | 41.28 | PB | +2.800 |
| 11 | Amy Acosta (Neo) | ECU | 41.79 | NR | +3.310 |
| 12 | Jenni Kukkonen (Senior) | FIN | 42.01 | | +3.530 |
| 13 | Karen Siatama (A2) | COL | 42.20 | PB | +3.720 |
| 14 | Shruti Kotwal (M30) | IND | 42.29 | | +3.810 |
| 15 | Isabela Vargas Rodriguez (A1) | COL | 43.05 | | +4.570 |
| 16 | Mia Manganello (Senior) | USA | 43.39 | | +4.910 |
| 17 | Evie Forrer (B1) | USA | 43.78 | | +5.300 |
| 18 | Cynthia Jennings (Senior) | USA | 44.33 | | +5.850 |