| 1 | Kimi Goetz (Senior) | USA | 37.89 | | - |
| 2 | McKenzie Browne (Senior) | USA | 38.89 | SB | +1.000 |
| 3 | Sofia Thorup (Senior) | DEN | 39.11 | | +1.220 |
| 4 | Arianna Fontana (Senior) | ITA | 39.35 | PB | +1.460 |
| 5 | Lindsey Woodbury (Neo) | USA | 41.34 | | +3.450 |
| 6 | Emma de Bock (B1) | USA | 41.58 | SB | +3.690 |
| 7 | Ilsa Melin Sandvig Shobe (Senior) | USA | 41.70 | | +3.810 |
| 8 | Marley Soldan (A1) | USA | 41.81 | | +3.920 |
| 9 | Laura Michelle Rodriguez (A2) | COL | 42.27 | SB | +4.380 |
| 10 | Natalia Valencia (Senior) | COL | 42.88 | | +4.990 |
| 11 | Ella Teeples (B1) | USA | 43.15 | SB | +5.260 |
| 12 | Shruti Kotwal (M30) | IND | 43.33 | | +5.440 |
| 13 | Leah Choi (B2) | USA | 43.90 | | +6.010 |
| 14 | Kaitlyn Lee (B1) | USA | 44.95 | | +7.060 |
| 15 | Karen Lizeth Siatama Valencia (A2) | COL | 45.32 | | +7.430 |
| 16 | Aileen Oh (C1) | USA | 45.71 | | +7.820 |
| 17 | Elisha Lee (C1) | USA | 46.07 | PB | +8.180 |
| 18 | Audrey Han (B2) | USA | 46.28 | | +8.390 |
| 19 | Clare Jeong (Senior) | USA | 47.29 | | +9.400 |
| 20 | Juliette Song (C1) | USA | 47.36 | PB | +9.470 |
| 21 | Allison La (C1) | USA | 47.58 | | +9.690 |
| 22 | Mariana Acero Pineda (C2) | COL | 48.13 | | +10.240 |
| 23 | Kaylin La (C1) | USA | 48.98 | | +11.090 |
| 24 | Chloe Kim (D1) | USA | 49.20 | PB | +11.310 |
| 25 | Seongsil Scarlet Heo (M30) | KOR | 49.50 | | +11.610 |
| 26 | Kathy Feinberg (M60) | USA | 57.42 | SB | +19.530 |
| 27 | Atira Meacham (E2) | USA | 1:01.68 | PB | +23.790 |