| 1 | Lindsey Woodbury (Neo) | USA | 41.17 | SB | - |
| 2 | Ilsa Melin Sandvig Shobe (Senior) | USA | 41.82 | | +0.650 |
| 3 | Natalia Valencia (Senior) | COL | 42.49 | | +1.320 |
| 4 | Larissa Paes (Senior) | BRA | 43.17 | | +2.000 |
| 5 | Leah Choi (B2) | USA | 43.57 | SB | +2.400 |
| 6 | Karen Lizeth Siatama Valencia (A2) | COL | 43.80 | | +2.630 |
| 7 | Laura Michelle Rodriguez (A2) | COL | 44.30 | | +3.130 |
| 8 | Kaitlyn Lee (B1) | USA | 44.86 | | +3.690 |
| 9 | Aileen Oh (C1) | USA | 45.49 | | +4.320 |
| 10 | Audrey Han (B2) | USA | 45.94 | SB | +4.770 |
| 11 | Elisha Lee (C1) | USA | 46.13 | | +4.960 |
| 12 | Allison La (C1) | USA | 46.92 | PB | +5.750 |
| 13 | Clare Jeong (Senior) | USA | 46.93 | SB | +5.760 |
| 14 | Mariana Acero Pineda (C2) | COL | 47.77 | SB | +6.600 |
| 15 | Kaylin La (C1) | USA | 48.54 | PB | +7.370 |
| 16 | Seongsil Scarlet Heo (M30) | KOR | 48.68 | PB | +7.510 |
| 17 | Chloe Kim (D1) | USA | 50.55 | | +9.380 |
| 18 | Kathy Feinberg (M60) | USA | 57.85 | | +16.680 |
| 19 | Atira Meacham (E2) | USA | 1:02.45 | | +21.280 |
| 20 | Juliette Song (C1) | USA | 1:05.70 | | +24.530 |
| DQ | Marley Soldan (A1) | USA | DQ | | - |