| 1 | Renske Winters (M40) | NED | 44.13 | | - |
| 2 | Gerdien Meijer-Verweij (M50) | NED | 44.21 | | +0.080 |
| 3 | Kaska Rogulska (M45) | NED | 44.37 | | +0.240 |
| 4 | Suzanne Mulder (M30) | NED | 44.66 | | +0.530 |
| 5 | Jeanette Hutterd ten Hag (M40) | NED | 44.92 | | +0.790 |
| 6 | Monique Boerema (M55) | NED | 45.44 | | +1.310 |
| 7 | Janette de Groot-Bron (M40) | NED | 46.27 | | +2.140 |
| 8 | Shirley Jaarsma (M30) | NED | 46.60 | | +2.470 |
| 9 | Sandra Bijlert-Burgerhout (M35) | NED | 47.26 | | +3.130 |
| 10 | Karin Terhürne (M45) | NED | 47.46 | SB | +3.330 |
| 11 | Ciska Stark (M50) | NED | 47.77 | | +3.640 |
| 12 | Marlies.64 de Jong (M50) | NED | 47.82 | | +3.690 |
| 13 | Marianne de Neeling (M55) | NED | 48.03 | | +3.900 |
| 14 | Noor van der Ster (M60) | NED | 48.37 | | +4.240 |
| 15 | Anita Smit-Groot (M50) | NED | 48.38 | | +4.250 |
| 16 | Rian Buitendijk (M45) | NED | 48.94 | | +4.810 |
| 17 | Barbara Heerschop (M60) | NED | 49.06 | | +4.930 |
| 18 | Tialda Gerritsma (M50) | NED | 50.24 | SB | +6.110 |
| 19 | Ingrid Ruijgrok (M50) | NED | 53.49 | | +9.360 |